Stereospecific analysis of triglycerides
Stereospecific analysis of triglycerides
复制标题
甘油三酯的立体定向分析
DOI：
10.1007/bf02531179
复制
发表时间：
1971
期刊：
影响因子：
1.9
通讯作者：
H. Brockerhoff
中科院分区：
文献类型：
--
作者：
H. Brockerhoff
立体特异性分析确定甘油三酯的脂肪酸如何分布在甘油的三个不同位置。特殊的问题是甘油的I-1位和L-3位的区别。在目前已知的方法中，甘油三酯首先通过脂肪酶的作用或通过格氏试剂降解而被降解为双甘油三酯的混合物。然后，在立体专一性酶的帮助下，双甘油酯异构体被分解，所述立体专一性酶可以是双甘油酯酶，也可以是磷脂酶(在双甘油酯转化为磷脂之后)。然后可以分析或计算甘油上每个位置的脂肪酸组成。成功的立体专一性分析的关键是通过甘油三酯的真正随机降解来制备具有代表性的双甘油三酯混合物。格氏降解法是最可靠的方法，但它并不总是适用的，它伴随着一些甘油的异构化。这种方法还有改进的余地。对天然脂肪的分析表明，它们大多是不对称的，即位置1的脂肪酸组成与位置3明显不同。脂肪酸分布的几个规律已变得明显。
Stereospecific analysis determines how the fatty acids of triglycerides are distributed over the three different positions of the glycerol. The special problem is the differentiation of position I‐1 and L‐3 of glycerol. In the presently known methods, triglycerides are first degraded to mixtures of diglycerides, either by the action of a lipase or by degradation with a Grignard reagent. The isomeric diglycerides are then resolved with the help of a stereospecific enzyme, either a diglyceride kinase or (after conversion of the diglycerides to phospholipids) a phospholipase. It is then possible to analyze or calculate the fatty acid composition for each position on the glycerol. The key to a successful stereospecific analysis is the preparation of a representative diglyceride mixture by a truly random degradation of the triglyceride. The Grignard degradation is the most reliable method, but it is not always applicable, and it is accompanied by some isomerization of glycerides. There is room for improvement in the method. Analyse of natural fats have shown most of them to be asymmetric, i.e., the composition of fatty acids in position 1 differs markedly from that of position 3. Several rules of fatty acid distribution have become apparent.