Response of the microaerophilic Bifidobactetium species, B-boum and B-thermophilum, to oxygen
Response of the microaerophilic Bifidobactetium species, B-boum and B-thermophilum, to oxygen
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DOI：
10.1128/aem.01216-06
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发表时间：
2006-10-01
影响因子：
4.4
通讯作者：
Niimura, Youichi
中科院分区：
文献类型：
--
作者：
Kawasaki, Shinji;Mimura, Tsuyoshi;Niimura, Youichi
我们使用液体振荡培养物在不同的0（2）浓度下研究了0（2）对双歧杆菌物种的影响。虽然我们选择的大多数双歧杆菌属的敏感性为0（2），但有两个菌种，R boum和B。thermophilum，表现出微需氧特征。B的生长。两歧和B.在高O2条件下，伴随着H2 O2在培养基中的积累，longum被抑制，并且通过向培养基中添加过氧化氢酶来恢复生长。B。boum和B.嗜热菌生长良好，即使在20%的O-2条件下没有H2 O2积累，生长刺激相比缺氧生长。B细胞提取液中主要检测到H2O形成型NADH氧化酶活性。boum和嗜热R. thermophilum在酸性反应条件（pH 5.0 ~ 6.0）下进行。
We investigated the effects of 0(2) on Bifidobacterium species using liquid shaking cultures under various 0(2) concentrations. Although most of the Bifidobacterium species we selected showed 0(2) sensitivity, two species, R boum and B. thermophilum, demonstrated microaerophilic profiles. The growth of B. bifidum and B. longum was inhibited under high-O-2 conditions accompanied by the accumulation of H2O2 in the medium, and growth was restored by adding catalase to the medium. B. boum and B. thermophilum grew well even under 20% O-2 conditions without H2O2 accumulation, and growth was stimulated compared to anoxic growth. H2O-forming NADH oxidase activities were detected dominantly in cell extracts of B. boum and R thermophilum under acidic reaction conditions (pH 5.0 to 6.0).