Proteosomes, emulsomes, and cholera toxin B improve nasal Immunogenicity of human immunodeficiency virus gp160 in mice: Induction of serum, intestinal, vaginal, and lung IgA and IgG
Proteosomes, emulsomes, and cholera toxin B improve nasal Immunogenicity of human immunodeficiency virus gp160 in mice: Induction of serum, intestinal, vaginal, and lung IgA and IgG
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DOI：
10.1093/infdis/175.2.292
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发表时间：
1997-02-01
影响因子：
6.4
通讯作者：
Birx, DL
中科院分区：
文献类型：
--
作者：
Lowell, GH;Kaminski, RW;Birx, DL
用与蛋白体复合的人免疫缺陷病毒（HIV）rgp 160鼻内免疫小鼠，与用盐水中未复合的rgp 160免疫相比，提高了抗gp 160血清伊加和IgG滴度，增加了识别的gp 160肽的数量，并刺激了抗gp 160肠道伊加，这些增强的响应在生物粘附性纳米乳液（乳胶体）或霍乱毒素B亚单位（CT B）加入到蛋白体-rgp 160疫苗中。用蛋白体鼻内免疫后，在阴道分泌物和粪便提取物中诱导抗gp 160 IgE和伊加，rgp 160在盐水中或与乳胶体一起递送，未复合的rgp 160与乳胶体或CTB的制剂也增强了血清和选择的粘膜伊加应答，诱导血清、阴道、支气管、肠，鼻内蛋白酶体检测粪便伊加和IgG-rgp 160疫苗在盐水中或与乳胶体或CTB一起递送对于粘膜疫苗开发是令人鼓舞的，以帮助控制HIV传播和AIDS的传播。
Intranasal immunization of mice with human immunodeficiency virus (HIV) rgp160 complexed to proteosomes improved anti-gp160 serum IgA and IgG titers, increased the number of gp160 peptides recognized, and stimulated anti-gp160 intestinal IgA compared with immunization with uncomplexed rgp160 in saline, These enhanced responses were especially evident when either a bioadhesive nanoemulsion (emulsomes) or cholera toxin B subunit (CTB) was added to the proteosome-rgp160 vaccine, Furthermore, anti-gp160 Ige and IgA in vaginal secretions and fecal extracts were induced after intranasal immunization with proteosome-rgp160 delivered either in saline or with emulsomes, Formulation of uncomplexed rgp160 with emulsomes or CTB also enhanced serum and selected mucosal IgA responses, Induction of serum, vaginal, bronchial, intestinal, and fecal IgA and IgG by intranasal proteosome-rgp160 vaccines delivered in saline or with emulsomes or CTB is encouraging for mucosal vaccine development to help control the spread of HIV transmission and AIDS.