Enhanced transglycosylation activity of an Endo-F3 mutant by ligand-directed localization
Enhanced transglycosylation activity of an Endo-F3 mutant by ligand-directed localization
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通过配体定向定位增强 Endo-F3 突变体的转糖基化活性
DOI：
10.1039/d2ob00030j
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Wei Huang
中科院分区：
文献类型：
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作者：
Xiangman Zou;Zhi Liu;Liya Liu;Wei Shi;Wanzhen Li;Zifen Guo;Feng Tang;Wei Huang
通过与Fc亲和标签化学武装开发了邻近定向的Endo-F3 D165 A，其对IgG表现出优异的转糖基化活性，表明通过化学工具增强酶活性的有希望的策略。
Proximity-directed Endo-F3 D165A was developed by chemically arming with an Fc affinity tag, which exhibited excellent transglycosylation activities towards IgG, indicating a promising strategy for enzyme activity enhancement by chemical tools.