Mechanical manipulation of bone and cartilage cells with 'optical tweezers'
Mechanical manipulation of bone and cartilage cells with 'optical tweezers'
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DOI：
10.1016/s0014-5793(99)01169-2
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发表时间：
1999-10-01
期刊：
影响因子：
3.5
通讯作者：
El Haj, AJ
中科院分区：
文献类型：
--
作者：
Walker, LM;Holm, Å;El Haj, AJ
单光束光学梯度阱（光镊）使用单束激光来非侵入性地操纵微观粒子。将施加约7倍pN力的光镊应用于培养中的单个骨和软骨来源的细胞，并使用Fluo-3标记观察细胞内钙水平的变化。人源性成骨细胞对光学镊子的反应是立即增加[Ca2+](i)，通过添加钙通道阻滞剂硝苯地平来抑制，施加于细胞不同区域的力导致不同的反应。[Ca2+](i)响应负荷的升高在大鼠股骨源性成骨细胞中较低，而在原代软骨细胞和骨细胞系（MLO Y4）中不明显。(C) 1999年欧洲生化学会联合会。
The single beam optical gradient trap (optical tweezers) uses a single beam of laser light to non-invasively manipulate microscopic particles. Optical tweezers exerting a force of approximately 7 pN were applied to single bone and cartilage derived cells in culture and changes in intracellular calcium levels were observed using Fluo-3 labelling. Human derived osteoblasts responded to optical tweezers with an immediate increase in [Ca2+](i) that was inhibited by the addition of a calcium channel blocker nifedipine, Force applied to different regions of cells resulted in a variable response. [Ca2+](i) elevation in response to load was lower in rat femur derived osteoblasts, and not apparent in primary chondrocytes and the osteocytic cell line (MLO Y4). (C) 1999 Federation of European Biochemical Societies.