Central leptin treatment modulates brain glucosensing function and peripheral energy metabolism of rainbow trout
Central leptin treatment modulates brain glucosensing function and peripheral energy metabolism of rainbow trout
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DOI：
10.1016/j.peptides.2010.02.026
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发表时间：
2010-06-01
期刊：
影响因子：
3
通讯作者：
Soengas, Jose L.
中科院分区：
文献类型：
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作者：
Aguilar, Ariel J.;Conde-Sieira, Marta;Soengas, Jose L.
本研究的目的是为瘦素对虹鳟鱼下丘脑和后脑葡萄糖敏感能力的可能调节作用提供证据。在第一个实验中，给鳟鱼ICV注射单独的生理盐水或含有增加剂量的瘦素（0.3-30 μ g μ l（-1））。瘦素一般诱导下丘脑和后脑中与葡萄糖传感相关的参数的剂量依赖性变化（增加的糖原生成和糖酵解潜力以及增加的GK活性，以及参与葡萄糖传感反应的基因的mRNA水平增加）与高血糖条件下发生的那些一致，高血糖条件是已知产生厌食症的情况。在第二个实验中观察到瘦素在我们实验条件下的厌食作用。在第三个实验中测试瘦素作用的特异性，其中向鳟鱼ICV注射盐水，或单独的瘦素，或瘦素加已知在哺乳动物中抑制瘦素信号传导途径的试剂。这些结果提示瘦素对葡萄糖敏感系统的中枢作用可能与JAK/STAT和IRS-PI（3）K通路有关。最后，我们还提供了证据表明，中枢瘦素治疗的外周效应（增加肝糖原分解潜力），这可能与交感神经活性增加有关。(C)2010年爱思唯尔公司All rights reserved.
The aim of the present study was to obtain evidence for the possible modulatory effect of leptin on glucosensing capacity in hypothalamus and hindbrain of rainbow trout. In a first experiment, trout were injected ICV with saline alone or containing increased doses of leptin (0.3-30 mu g mu l(-1)). Leptin induced in general in both hypothalamus and hindbrain dose-dependent changes in parameters related to glucosensing (increased glycogenic and glycolytic potentials together with increased GK activity, and increased mRNA levels of genes involved in glucosensing response) compatible with those occurring under hyperglycemic conditions, a situation that is known to produce anorexia. The anorectic action of leptin in our experimental conditions was observed in a second experiment. The specificity of leptin action was tested in a third experiment in which trout were injected ICV with saline, or leptin alone, or leptin plus agents known to inhibit leptin signaling pathways in mammals. The results obtained suggest that the central action of leptin on glucosensing system can be related to the JAK/STAT and IRS-PI(3) K pathways. Finally, we also provide evidence for a peripheral effect of central leptin treatment (increased liver glycogenolytic potential), which could be associated with increased sympathetic activity. (C) 2010 Elsevier Inc. All rights reserved.