Neural regulation of inflammation: no neural connection from the vagus to splenic sympathetic neurons
Neural regulation of inflammation: no neural connection from the vagus to splenic sympathetic neurons
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DOI：
10.1113/expphysiol.2011.061531
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发表时间：
2012-11-01
影响因子：
2.7
通讯作者：
McAllen, R. M.
中科院分区：
文献类型：
--
作者：
Bratton, B. O.;Martelli, D.;McAllen, R. M.
炎性反射通过从中枢神经系统到淋巴器官（特别是脾）的传出神经连接起作用，其抑制炎性细胞因子的产生。已显示刺激传出迷走神经以依赖于脾和脾神经的方式抑制炎症。迷走神经不支配脾脏，因此建议迷走神经节前神经元与脾脏交感神经节后神经元之间存在突触连接。我们在老鼠身上测试了这个想法。在预备手术中，将顺行示踪剂DiI注射到双侧迷走神经背侧运动核中，并将逆行示踪剂Fast Blue注射到脾脏中。在79周后的组织学分析中，883个神经元用固蓝从脾脏逆行标记，如下：89%在肾上腺神经节中（左侧65%，右侧24%）; 11%在左侧腹腔神经节中;但在右侧腹腔或任何一个上级肠系膜神经节中都没有。迷走神经末梢顺行标记DiI是常见的腹腔，但稀疏的肾上腺神经节，共聚焦分析显示，没有假定的突触连接与任何快蓝标记的细胞在任何神经节。在麻醉大鼠的电生理实验表明，迷走神经传出刺激对脾神经活动或对肾上腺神经节中记录的15个单个脾投射神经元没有影响。总之，这些发现表明，大鼠迷走神经传出神经元既不与脾交感神经元突触，也不驱动它们的持续活动。
The inflammatory reflex acts through efferent neural connections from the central nervous system to lymphoid organs, particularly the spleen, that suppress the production of inflammatory cytokines. Stimulation of the efferent vagus has been shown to suppress inflammation in a manner dependent on the spleen and splenic nerves. The vagus does not innervate the spleen, so a synaptic connection from vagal preganglionic neurons to splenic sympathetic postganglionic neurons was suggested. We tested this idea in rats. In a preparatory operation, the anterograde tracer DiI was injected bilaterally into the dorsal motor nucleus of vagus and the retrograde tracer Fast Blue was injected into the spleen. On histological analysis 79 weeks later, 883 neurons were retrogradely labelled from the spleen with Fast Blue as follows: 89% in the suprarenal ganglia (65% left, 24% right); 11% in the left coeliac ganglion; but none in the right coeliac or either of the superior mesenteric ganglia. Vagal terminals anterogradely labelled with DiI were common in the coeliac but sparse in the suprarenal ganglia, and confocal analysis revealed no putative synaptic connection with any Fast Blue-labelled cell in either ganglion. Electrophysiological experiments in anaesthetized rats revealed no effect of vagal efferent stimulation on splenic nerve activity or on that of 15 single splenic-projecting neurons recorded in the suprarenal ganglion. Together, these findings indicate that vagal efferent neurons in the rat neither synapse with splenic sympathetic neurons nor drive their ongoing activity.