Total sleep deprivation impairs the encoding of trace-conditioned memory in the rat
Total sleep deprivation impairs the encoding of trace-conditioned memory in the rat
复制标题
DOI：
10.1016/j.nlm.2011.01.009
复制
发表时间：
2011-03-01
影响因子：
2.7
通讯作者：
Jha, Sushi K.
中科院分区：
文献类型：
--
作者：
Chowdhury, Ananya;Chandra, Ruchi;Jha, Sushi K.
睡眠可能有助于巩固某些记忆的信息，尽管它在巩固痕迹条件记忆方面的好处仍然难以捉摸。本研究探讨了睡眠剥夺对雄性Wistar大鼠痕迹学习的影响。训练大鼠进行痕迹条件化训练，并将头部进入液体分配器的次数作为痕迹学习的结果测量。对于训练和测试，在五个会话中提供75个条件刺激（CS）（光）和非条件刺激（US）（果汁）的呈现（15个呈现/会话;具有5分钟会话间隙）。CS和US刺激的持续时间分别为15和20 s，刺激之间的跟踪延迟为5 s，每次呈现之间的条件延迟为20 s。训练结束后将动物随机分为睡眠剥夺（SD）组（n = 8）、非睡眠剥夺（NSD）组（n = 8）和应激对照组（n = 5）。NSD组和对照组动物不受干扰，SD组动物训练后剥夺睡眠6 h。在接下来的几天里，我们检查了轨迹条件任务的学习。我们观察到，在测试日，SD大鼠比NSD和对照组少戳约63%（p < 0.001）以获得汁液。此外，NSD大鼠在训练日和测试日的头部进入次数呈显著正相关，而SD大鼠没有表现出显著的相关性。结果表明，SD动物有困难，联想CS与US，并建议睡眠剥夺后不久，训练损害的痕迹记忆的编码。(C)2011 Elsevier Inc. All rights reserved.
Sleep may help consolidate the information of certain memories, though its benefits in the consolidation of trace-conditioned memory still remain elusive. We investigated the effect of sleep deprivation on trace learning in male wistar rats. Rats were trained for trace conditioning and the number of head entries into liquid dispenser was accounted as an outcome measure of trace-learning. For training and testing, 75 presentations of conditioned stimulus (CS) (light) and unconditioned stimulus (US) (juice) were offered in five sessions (15 presentations/session; with 5 min inter-session gap). The duration of CS and US stimuli were 15 and 20 s respectively, with 5 s trace delay between stimuli and 20 s condition delay between each presentation. The animals were divided randomly into three groups soon after training, sleep deprived (SD) (n = 8), non-SD (NSD) (n = 8) and stress control (n = 5) groups. The animals of NSD and control groups were left undisturbed, while SD animals were sleep deprived for 6 h after training. The learning of trace-conditioned task was examined on following days. We observed that SD rats poked approximately 63% less than NSD and control groups (p < 0.001) to obtain juice on testing day. Also, the NSD rats exhibited significant positive correlation in number of head entries during the training and testing days; while the SD rats showed no significant correlation. The results demonstrate that SD animals had difficulties to associate CS with US and suggest that sleep deprivation soon after training impairs the encoding of trace memory. (C) 2011 Elsevier Inc. All rights reserved.