Quercetin is equally or more effective than resveratrol in attenuating tumor necrosis factor-α-mediated inflammation and insulin resistance in primary human adipocytes
Quercetin is equally or more effective than resveratrol in attenuating tumor necrosis factor-α-mediated inflammation and insulin resistance in primary human adipocytes
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DOI：
10.3945/ajcn.2010.29807
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发表时间：
2010-12-01
影响因子：
7.1
通讯作者：
McIntosh, Michael K.
中科院分区：
文献类型：
--
作者：
Chuang, Chia Chi;Martinez, Kristina;McIntosh, Michael K.
背景 槲皮素和反式白藜芦醇 (trans RSV) 是植物多酚，据报道可减少与肥胖相关的炎症或胰岛素抵抗。最近我们发现，含有槲皮素和反式 RSV 的葡萄粉提取物可减轻人类脂肪细胞和巨噬细胞的炎症标志物以及人类脂肪细胞中的胰岛素抵抗。但是，我们不知道槲皮素和反式 RSV 如何单独影响 这些结果目的本研究的目的是检查槲皮素和反式 RSV 在用肿瘤坏死因子 α (TNF α) 处理的人脂肪细胞原代培养物中预防炎症或胰岛素抵抗的程度，TNF α 是肥胖糖尿病个体血浆和脂肪组织中升高的炎症细胞因子设计对人脂肪细胞培养物进行预处理 用槲皮素和反式 RSV 进行治疗，然后用 TNF α 治疗 随后测量了炎症和胰岛素抵抗的基因和蛋白质标记物 结果 槲皮素和反式 RSV 在较小程度上减弱了 TNF α 诱导的炎症基因的表达，例如白细胞介素 (IL) 6 IL I β IL 8 和单核细胞趋化蛋白 1 (MCP I) 的表达以及 IL 6 IL 8 和 MCP 1 槲皮素减弱 TNF α 介导的细胞外信号相关激酶和 c Jun NH2 末端激酶的磷酸化，而反式 RSV 仅减弱 c Jun NH2 末端激酶的磷酸化 槲皮素和反式 RSV 减弱 TNF α 介导的 c Jun 磷酸化和抑制蛋白的降解 KB 蛋白 Quercenn 但不反式 RSV 降低 TNF a 诱导的核因子 kappa B 转录活性 槲皮素和反式 RSV 减弱 TNF α 介导的过氧化物酶体增殖物激活受体 y (PPAR γ) 和 PPAR γ 靶基因以及 PPAR γ 蛋白浓度和转录活性的抑制 槲皮素可抑制 TNF α 介导的胰岛素受体底物 1 和蛋白酪氨酸磷酸酶 kappa B 基因表达的 senne 磷酸化以及对胰岛素刺激的葡萄糖摄取的抑制，而反式 RSV 仅阻止 TNF α 介导的胰岛素受体底物的 senne 磷酸化。 减轻原代人脂肪细胞中 TNF α 介导的炎症和胰岛素抵抗 Am J Chn Ntttr 2010 92 1511-21
Background Quercetin and trans resveratrol (trans RSV) are plant polyphenols reported to reduce inflammation or insulin resistance associated with obesity Recently we showed that grape powder extract which contains quercetin and trans RSV attenuates markers of inflammation in human adipocytes and macrophages and insulin resistance in human adipocytes However we do not know how quercetin and trans RSV individually affected these outcomesObjective The aim of this study was to examine the extent to which quercetin and trans RSV prevented inflammation or insulin resistance in primary cultures of human adipocytes treated with tumor necrosis factor alpha (TNF alpha) an inflammatory cytokine elevated in the plasma and adipose tissue of obese diabetic individualsDesign Cultures of human adipocytes were pretreated with quercetin and trans RSV followed by treatment with TNF alpha Subsequently gene and protein markers of inflammation and insulin resistance were measuredResults Quercetin and to a lesser extent trans RSV attenuated the TNF a induced expression of inflammatory genes such as interleukin (IL) 6 IL I beta IL 8 and monocyte chemoattractant protein 1 (MCP I) and the secretion of IL 6 IL 8 and MCP 1 Quercetin attenuated TNF alpha-mediated phosphorylation of cxtracellular signal related kinase and c Jun NH2 terminal kinase whereas trans RSV attenuated only c Jun NH2 terminal kinase phosphorylation Querce tin and trans RSV attenuated TNF alpha-mediated phosphorylation of c Jun and degradation of inhibitory KB protein Quercenn but not trans RSV decreased TNF a induced nuclear factor kappa B transcnp tional activity Quercetin and trans RSV attenuated the TNF alpha-mediated suppression of peroxisome proliferator activated receptor y (PPAR gamma) and PPAR gamma target genes and of PPAR gamma protein concen trations and transcriptional activity Quercetin prevented the TNF alpha-mediated senne phosphorylation of insulin receptor substrate 1 and protein tyrosine phosphatase kappa B gene expression and the suppression of insulin stimulated glucose uptake whereas trans RSV prevented only the TNF alpha-mediated senne phosphorylation of insulin receptor substrate IConclusion These data suggest that quercetin is equally or more effective than trans RSV in attenuating TNF alpha-mediated inflamma tion and insulin resistance in primary human adipocytes Am J Chn Ntttr 2010 92 1511-21