miR-125b inhibits osteoblastic differentiation by down-regulation of cell proliferation
miR-125b inhibits osteoblastic differentiation by down-regulation of cell proliferation
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DOI：
10.1016/j.bbrc.2008.01.073
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发表时间：
2008-04-04
影响因子：
3.1
通讯作者：
Okazaki, Yasushi
中科院分区：
文献类型：
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作者：
Mizuno, Yosuke;Yagi, Ken;Okazaki, Yasushi
尽管有多种microRNA调控细胞分化和增殖，但迄今为止还没有报道miRNA在成骨细胞分化的调控中发挥重要作用。在这里，我们描述了miR-125 b在小鼠间充质干细胞（ST 2）成骨分化中的作用，通过调节细胞增殖。ST 2细胞中miR-125 b的表达呈时间依赖性增加，BMP-4诱导的成骨分化的ST 2细胞中miR-125 b表达的增加减弱。转染外源性miR-125 b可抑制ST 2细胞的增殖并导致成骨细胞分化受到抑制。相反，当内源性miR-125 b被其反义RNA分子转染阻断时，BMP-4处理后碱性磷酸酶活性升高。这些结果强烈表明，miR-125 b通过调节细胞增殖参与成骨细胞分化。(C)2008年爱思唯尔公司All rights reserved.
Although various microRNAs regulate cell differentiation and proliferation, no miRNA has been reported so far to play an important role in the regulation of osteoblast differentiation. Here we describe the role of miR-125b in osteoblastic differentiation in mouse mesenchymal stem cells, ST2, by regulating cell proliferation. The expression of miR-125b was time-dependently increased in ST2 cells, and the increase in miR-125b expression was attenuated in osteoblastic-differentiated ST2 cells induced by BMP-4. The transfection of exogenous miR-125b inhibited proliferation of ST2 cells and caused inhibition of osteoblastic differentiation. In contrast, when the endogenous miR-125b was blocked by transfection of its antisense RNA molecule, alkaline phosphatase activity after BMP-4 treatment was elevated. These results strongly suggest that miR-125b is involved in osteoblastic differentiation through the regulation of cell proliferation. (C) 2008 Elsevier Inc. All rights reserved.