Evidence for production of oxidizing radicals by the particulate O-2-forming system from human neutrophils.
Evidence for production of oxidizing radicals by the particulate O-2-forming system from human neutrophils.
复制标题
人类中性粒细胞的颗粒 O-2 形成系统产生氧化自由基的证据。
作者：
A. Tauber;T. Gabig;B. Babior
发现人体中性粒细胞形成的颗粒o- 2形成系统将甲基和2-酮-4-甲基硫丁酸（KMB）氧化为乙烯，表明该系统形成了强氧化自由基。乙烯的产生被自由基清除剂苯甲酸酯、乙醇和甘露醇抑制，这与这一解释一致。乙烯的产生也被超氧化物歧化酶急剧减少，这意味着O-2是氧化自由基的前体。在我们的系统中，过氧化氢酶仅部分抑制甲基或KMB的乙烯生成，这表明氧化自由基至少部分是由O-2与H2O2以外的化合物反应产生的。我们提出，在中性粒细胞中，氧化自由基是在O-2和过氧化物之间的反应中形成的，根据以下公式：O-2 + ROOH生成RO。+ OH- + O2，其中ROOH可以是过氧化氢、烷基过氧化氢或酰基过氧化氢（即过氧酸）。
The particulate O-2-forming system from human neutrophils was found to oxidize methional and 2-keto-4-methylthiobutyric acid (KMB) to ethylene, indicating the formation by this system of strongly oxidizing radicals. Conforming this interpretation was the observation that ethylene production was inhibited by the radical scavengers benzoate, ethanol, and mannitol. Ethylene production was also sharply reduced by superoxide dismutase, implicatin O-2 as a precursor of oxidizing radicals. In our system catalase only partially inhibited ethylene generation from either methional or KMB, suggesting that oxidizing radicals are generated at least in part by the reacton of O-2 with compounds other than H2O2. We propose that in neutrophils oxidizing radicals are formed in a reaction between O-2 and a peroxide according to the following equation: O-2 + ROOH leads to RO . + OH- + O2, in which ROOH may be hydrogen peroxide, an alkyl peroxide, or an acyl peroxide (i.e., a peroxy acid).