Biomimetic synthesis of vaterite CaCO_3 microspheres under threonine for preparation of pH-responsive antibacterial biofilm
Biomimetic synthesis of vaterite CaCO_3 microspheres under threonine for preparation of pH-responsive antibacterial biofilm
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DOI：
10.1557/jmr.2020.178
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发表时间：
2020-07
影响因子：
2.7
通讯作者：
Tingyu Yang;Yuehua Wu;Xiaoqing Yue;Cuiyan Wang;Jianbin Zhang
中科院分区：
文献类型：
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作者：
Tingyu Yang;Yuehua Wu;Xiaoqing Yue;Cuiyan Wang;Jianbin Zhang
针对特定细菌生长环境，合成具有特定功能的抗菌生物材料，对实现有效杀菌、减少耐药菌具有重要意义。本文以生物矿化为研究对象，采用CO_2鼓泡法，以苏氨酸（Thr）为载体，一锅法制备了球形碳酸钙微球，并将其作为抗菌药物血根碱（SAN）的载体。球文石型CaCO 3微球对SAN类药物的载药量达到159.8mg/g，载药率为83.7%。首次利用pH响应性、SAN载药量高的球文石型碳酸钙微球构建了一种新型的SAN @碳酸钙（SAN@CaCO_3）有机-无机杂化抗菌生物膜。该膜具有细菌触发、pH响应的SAN释放特性，对金黄色葡萄球菌（S. aureus)具有良好的抗菌活性。同时，在真实的环境中也具有抗菌能力。7天，能显著抑制空气中细菌的附着和生长。生物矿化合成球文石型碳酸钙微球及其在构建pH响应性抗菌生物膜中的应用，在抗细菌感染、减少耐药菌产生方面具有广阔的应用前景。
The synthesis of antibacterial biomaterial with specific functions responsive to specific bacterial growth environments is of significant importance to achieve effective sterilization and reduce the resistant bacteria. Herein, inspired by biomineralization, we develop a one-pot, threonine (Thr)-mediated biomineralization method using a CO_2 bubbling procedure to green, simply and quickly prepare vaterite CaCO_3 microspheres as a platform for antibacterial Sanguinarine (SAN) delivery. The loading capacity of vaterite CaCO_3 microspheres for SAN drugs reached 159.8 mg/g, corresponding to the loading efficiency of 83.7%. And for the first time, a novel Sanguinarine@calcium carbonate (SAN@CaCO_3) organic–inorganic hybrid antibacterial biofilm was constructed by using vaterite CaCO_3 microspheres with pH-responsive and high SAN drug-loading. Importantly, the film showed bacteria-triggered, pH-responsive SAN release properties and strong bactericidal ability (96.19%) for Staphylococcus aureus ( S. aureus ). Meanwhile, it also had antibacterial capabilities in real environments. In 7 days, it can significantly inhibit the adhesion and growth of bacteria in the air. The biomineralized synthetic vaterite CaCO_3 microspheres and the application in the construction of pH-responsive antibacterial biofilm have bright future in resisting bacterial infections and reducing the production of resistant bacteria.