Classic Spotlight: How the Gram Stain Works.
Classic Spotlight: How the Gram Stain Works.
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经典聚焦：革兰氏染色的工作原理。
DOI：
10.1128/jb.00726-16
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发表时间：
2016
影响因子：
3.2
通讯作者：
O'Toole,GeorgeA
中科院分区：
文献类型：
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作者：
O'Toole,GeorgeA
革兰氏染色。这种染色剂最初是为了帮助区分组织中的细菌和宿主细胞而开发的，现在已经发展成为一种关键的检测方法，帮助临床医生决定应该使用哪种抗生素来治疗感染，因为革兰氏阳性和革兰氏阴性微生物对某些类别的抗菌剂表现出不同的反应。虽然没有提供任何系统发育信息，但紫色和粉红色染色反映了这两组生物体的不同生物学特性，并反映了许多临床相关微生物之间的关系，这些微生物由基于16 S rRNA基因的分析定义。通常认为革兰氏阳性生物体的厚细胞壁有助于在用乙醇处理时保留紫色结晶紫-碘复合物。在1983年《细菌学杂志》上的一篇文章中，Beveridge和Davies（1）使用了一种带有电子致密物质的革兰氏染色法，
Gram stain. This stain, originally developed to help distinguish bacteria from host cells in tissue, has evolved as a key assay to help clinicians decide which antibiotics should be used to treat infections, given that Gram-positive and Gram-negative organisms display differential responses to some classes of antimicrobial agents. While not providing any phylogenetic information, the purple versus pink staining mirrors the different biologies of these two groups of organisms and reflects relationships between many clinically relevant microbes as defined by 16S rRNA gene-based analyses.Since its development in the late 1800s, evidence has accumulated that the Gram stain differentiates between microbes based on their ultrastructure; typically the thick cell wall of Gram-positive organisms is credited with helping to retain the purple crystal violet-iodine complex upon treatment with ethanol. In a 1983 publication in the Journal of Bacteriology, Beveridge and Davies (1) used a variation of Gram stain with an electron-dense substi-