Lipogenesis from glucose-2- 14 C and acetate-1- 14 C in aorta.
Lipogenesis from glucose-2- 14 C and acetate-1- 14 C in aorta.
复制标题
主动脉中葡萄糖-2- 14 C 和乙酸-1- 14 C 的脂肪生成。
作者：
C. Howard
在正常和动脉粥样硬化兔子的外翻主动脉中用葡萄糖-2-(14)C 和乙酸-1-(14)C 测量脂肪生成。在正常主动脉和动脉粥样硬化主动脉中，更多的葡萄糖-2-(14)C 比乙酸-1-(14)C 掺入脂质中。来自葡萄糖-2-(14)C的放射性碳主要出现在甘油三酯和磷脂中，少量出现在胆固醇酯中。随着动脉粥样硬化的发生，掺入量几乎增加了三倍，大部分放射性位于甘油部分；放射性主要存在于甘油的碳2中。掺入磷脂和甘油三酯中的乙酸-1-(14)C约70%在脂肪酸中，其余部分在甘油酯-甘油中；胆固醇酯中 98% 的放射性位于脂肪酸部分。在动脉粥样硬化的发展过程中，与胆固醇酯的结合增加最多。乙酸酯-1-(14)C 和源自葡萄糖-2-(14)C 的 2 碳单元的脂肪酸合成相似，即主要从头合成具有 14 和 16 个碳原子的脂肪酸，以及延伸 18 个碳及更长的脂肪酸。
Lipogenesis was measured with glucose-2-(14)C and acetate-1-(14)C in the everted aortas of normal and atherosclerotic rabbits. More glucose-2-(14)C than acetate-1-(14)C was incorporated into lipids in both the normal and the atherosclerotic aorta. Radiocarbon from glucose-2-(14)C appeared mainly in triglycerides and phospholipids with a small amount in cholesteryl esters. Incorporation increased almost threefold with atherosclerosis, most of the radioactivity being in the glycerol moiety; radioactivity was predominantly in carbon 2 of glycerol. About 70% of the acetate-1-(14)C incorporated into phospholipids and triglycerides was in the fatty acids, and the remainder was in glyceride-glycerol; 98% of the radioactivity in cholesteryl esters was in the fatty acid moiety. Incorporation into cholesteryl esters was increased most during the development of atherosclerosis. Fatty acid synthesis was similar from both acetate-1-(14)C and the 2 carbon unit derived from glucose-2-(14)C, viz., predominantly de novo synthesis of fatty acids with 14 and 16 carbon atoms, and elongation for those of 18 carbons and longer.