Injectable alginate-microencapsulated canine adipose tissue-derived mesenchymal stem cells for enhanced viable cell retention.
Injectable alginate-microencapsulated canine adipose tissue-derived mesenchymal stem cells for enhanced viable cell retention.
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DOI：
10.1292/jvms.16-0456
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发表时间：
2017-03-18
期刊：
影响因子：
--
通讯作者：
Kang BJ
中科院分区：
文献类型：
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作者：
Koh E;Jung YC;Woo HM;Kang BJ
本研究的目的是建立海藻酸盐包裹的犬脂肪间充质干细胞(CASCs)的优化生产方案，并评价其在临床上的适用性，包括活性、增殖和体内细胞保留。海藻酸盐微球是通过振动技术形成的，可注射微球的生产是在标准方法下使用各种参数进行的。微珠的毒性在动物模型中进行了测试。体外检测微囊化CASCs的活性和增殖能力。将有或没有微囊化的HEK-293细胞注射到小鼠的皮下组织中，并使用体内生物发光成像进行跟踪，以评估移植细胞的保留情况。优化后的可注射微球大小均匀，直径约250微米。没有强有力的证据表明海藻酸盐珠具有体内毒性。细胞在包裹后仍然存活，并且有证据表明微囊内有体外增殖的迹象。体内生物发光成像显示，海藻酸盐微囊化提高了移植细胞的保留率，微囊化后的细胞在体内存活7天。胶囊化可增强活细胞在体内的保留，并可能代表一种潜在的策略，以提高干细胞的治疗效力和疗效。
The purpose of this study was to establish an optimized protocol for the production of alginate-encapsulated canine adipose-derived mesenchymal stem cells (cASCs) and evaluate their suitability for clinical use, including viability, proliferation and in vivo cell retention. Alginate microbeads were formed by vibrational technology and the production of injectable microbeads was performed using various parameters with standard methodology. Microbead toxicity was tested in an animal model. Encapsulated cASCs were evaluated for viability and proliferation in vitro. HEK-293 cells, with or without microencapsulation, were injected into the subcutaneous tissue of mice and were tracked using in vivo bioluminescent imaging to evaluate the retention of transplanted cells. The optimized injectable microbeads were of uniform size and approximately 250 µm in diameter. There was no strong evidence of in vivo toxicity for the alginate beads. The cells remained viable after encapsulation, and there was evidence of in vitro proliferation within the microcapsules. In vivo bioluminescent imaging showed that alginate encapsulation improved the retention of transplanted cells and the encapsulated cells remained viable in vivo for 7 days. Encapsulation enhances the retention of viable cells in vivo and might represent a potential strategy to increase the therapeutic potency and efficacy of stem cells.