Novel shuttle plasmid vehicles for Escherichia-Streptococcus transgeneric cloning.
Novel shuttle plasmid vehicles for Escherichia-Streptococcus transgeneric cloning.
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DOI：
10.1016/0378-1119(83)90176-2
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发表时间：
1983-11
期刊：
影响因子：
3.5
通讯作者：
Francis L. Marcrina;R. Evans;J. A. Tobian;D. Hartley;D. Clewell;K. Jones
中科院分区：
文献类型：
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作者：
Francis L. Marcrina;R. Evans;J. A. Tobian;D. Hartley;D. Clewell;K. Jones
描述了一种能够在大肠杆菌和血链球菌中复制的新型质粒载体。这个9.2kb的质粒命名为pVA 856，携带在大肠杆菌中表达的Cmr、Tcr和Emr决定簇。杆菌只有Emr决定簇在血链球菌中表达。pVA 856的Ccr和Tcr都可以被插入失活。该质粒提供了几种不同的切割-连接策略用于在E中克隆。大肠杆菌，随后在toS中引入嵌合体。sanguis。此外，我们修改了先前描述的E。coli-S.血液穿梭质粒[pVA 838; Macrina等人，Gene 19（1982）345-353]，因此它不能在血链球菌中复制。证明了这种质粒用于克隆和选择能够在血链球菌中自主复制的序列的实用性。
A novel plasmid vector that is able to replicate both inEscherichia coliand inStreptococcus sanguisis described. This 9.2-kb plasmid, designated pVA856, carries Cmr, Tcrand Emrdeterminants that are expressed inE. coli. Only the Emrdeterminant is expressed inS. sanguis. Both the Cmrand the Tcrof pVA856 may be insertionally inactivated. This plasmid affords several different cleavage-ligation strategies for cloning inE. colifollowed by subsequent introduction of chimeras in toS. sanguis. In addition, we have modified a previously describedE. coli-S. sanguisshuttle plasmid [pVA838; Macrina et al., Gene 19 (1982) 345–353], so that it is unable to replicate inS. sanguis. The utility of such a plasmid for cloning and selecting sequences enabling autonomous replication inS. sanguisis demonstrated.