Seawater transfer alters the intestinal microbiota profiles of Atlantic salmon (Salmo salar L.).
Seawater transfer alters the intestinal microbiota profiles of Atlantic salmon (Salmo salar L.).
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DOI：
10.1038/s41598-017-13249-8
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发表时间：
2017-10-24
影响因子：
4.6
通讯作者：
Martin SAM
中科院分区：
文献类型：
--
作者：
Dehler CE;Secombes CJ;Martin SAM
大西洋鲑鱼在从淡水迁移到海洋环境的过程中经历了巨大的生理变化。渗透调节适应是最关键的变化，需要鳃，肾和肠的功能适应。此外，在盐水环境中遇到了新的病原体，微生物和饮食项目，这表明肠道微生物群在向盐水移动后发生了重大变化。在这里，我们比较了肠道微生物群窝藏在大西洋鲑鱼淡水鱼（FW）保持在商业苏格兰淡水孵化场与他们的全同胞海水驯化（SW）后，通过16 S rRNA（V3-V4）高通量测序方法的远端腹直肌。无论是在丰富度还是多样性方面，FW中的α多样性和β多样性都显着高于SW。Metastats分析发现，与SW相比，FW特有的操作分类单位（OTU）数量更多，另外还有238个OTU的丰度显著不同。在100%的鱼类中鉴定出19个OTU的核心微生物群，这表明某些微生物在肠道内维持最低限度的功能。此外，我们表明，各自的微生物配置文件的独特性可以与KEGG途径，包括脂质和氨基酸代谢的显着差异。
Atlantic salmon undergo dramatic physiological changes as they migrate from freshwater to the marine environment. Osmoregulatory adaptation is the most crucial change, necessitating functional adaptations of the gills, kidney and intestine. Additionally, novel pathogens, microbes and dietary items are encountered in the saltwater environment, which suggests major changes in the intestinal microbiota following movement to saltwater. Here we compared the intestinal microbiota harboured in the distal digesta of Atlantic salmon freshwater fish (FW) kept in a commercial Scottish freshwater hatchery with that of their full-siblings after seawater acclimatisation (SW) by a 16S rRNA (V3-V4) high-throughput sequencing approach. Alpha- and beta-diversity were found significantly higher in FW compared to SW, both in terms of richness and diversity. Metastats analysis identified a higher number of Operational Taxonomic Units (OTUs) unique to FW compared to SW, with an additional 238 OTUs found at significantly different abundance. A core microbiota of 19 OTUs was identified in 100% of all fish, which indicates that certain microbes are maintained to fulfil minimal functions within the gut. Furthermore we show that the uniqueness of the respective microbial profiles can be correlated with significant differences in KEGG pathways including lipid and amino acid metabolism.
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影响因子：
4.3
作者：
Milligan-Myhre K;Small CM;Mittge EK;Agarwal M;Currey M;Cresko WA;Guillemin K
通讯作者：
Guillemin K
影响因子：
14.9
作者：
Klindworth A;Pruesse E;Schweer T;Peplies J;Quast C;Horn M;Glöckner FO
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Glöckner FO
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Beiko, Robert G.
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Holben, WE;Williams, P;Apajalahti, JHA
通讯作者：
Apajalahti, JHA
影响因子：
4.5
作者：
Krogdahl, Ashild;Sundby, Anne;Holm, Halvor
通讯作者：
Holm, Halvor