Growth stimulation in inflorescences of an Arabidopsis tubulin mutant under microgravity conditions in space
Growth stimulation in inflorescences of an Arabidopsis tubulin mutant under microgravity conditions in space
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DOI：
10.1111/plb.12099
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发表时间：
2014-01-01
期刊：
影响因子：
3.9
通讯作者：
Kamisaka, S.
中科院分区：
文献类型：
--
作者：
Hoson, T.;Soga, K.;Kamisaka, S.
皮层微管参与植物对超重力的抵抗，但其在抵抗1g重力中的作用仍然不确定。为了阐明这一点，我们在国际空间站Kibo模块上的细胞生物学实验设施中培养了拟南芥微管蛋白6突变体（tua 6），并分析了花序的生长和细胞壁机械特性。花序茎的生长刺激微重力条件下，与地面和在轨1g条件相比。微重力条件下的茎比1g条件下的茎长10-45%，其生长速率高15-55%。tua 6突变体的生长刺激程度往往高于野生型哥伦比亚。在微重力条件下，细胞壁的延伸性在花序的伸长区域显着高于对照，表明生长刺激是由细胞壁的修改。在地面和在轨1g控制之间的任何生长或细胞壁特性方面没有发现明显的差异。这些结果支持了皮层微管在植物抵抗重力中起重要作用的假设。
Cortical microtubules are involved in plant resistance to hypergravity, but their roles in resistance to 1g gravity are still uncertain. To clarify this point, we cultivated an Arabidopsis -tubulin 6 mutant (tua6) in the Cell Biology Experiment Facility on the Kibo Module of the International Space Station, and analyzed growth and cell wall mechanical properties of inflorescences. Growth of inflorescence stems was stimulated under microgravity conditions, as compared with ground and on-orbit 1g conditions. The stems were 10-45% longer and their growth rate 15-55% higher under microgravity conditions than those under both 1g conditions. The degree of growth stimulation tended to be higher in the tua6 mutant than the wild-type Columbia. Under microgravity conditions, the cell wall extensibility in elongating regions of inflorescences was significantly higher than the controls, suggesting that growth stimulation was caused by cell wall modifications. No clear differences were detected in any growth or cell wall property between ground and on-orbit 1g controls. These results support the hypothesis that cortical microtubules generally play an important role in plant resistance to the gravitational force.