Focal/multifocal and geographic retinal dysplasia in the dog-In vivo retinal microanatomy analyses
Focal/multifocal and geographic retinal dysplasia in the dog-In vivo retinal microanatomy analyses
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DOI：
10.1111/vop.12725
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发表时间：
2020-03-01
影响因子：
1.6
通讯作者：
Aguirre, Gustavo D.
中科院分区：
文献类型：
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作者：
Iwabe, Simone;Dufour, Valerie L.;Aguirre, Gustavo D.
目的研究获得性或遗传性视网膜发育不良犬视网膜皱褶和地图样病变的显微解剖学，材料与方法13只犬在全麻下用cSLO/sdOCT进行视网膜显微解剖学评价，其中2只患非遗传性多灶性视网膜皱褶，5只患遗传性多灶性视网膜皱褶（drd 1或drd 2），10只患地图样视网膜发育不良。视网膜从两个drd 2载体狗进行了检查，通过组织学和免疫组织化学（IHC）后，在体内imaging.Results视网膜褶皱是获得性局灶性/多灶性或地理视网膜发育不良的共同特点，是无法区分的结构与综合征眼骨骼发育不良，并代表外核层内陷和roxaminations可见sdOCT。在眼骨骼发育不良的杂合子犬中，褶皱形成簇，沿上级中央血管沿着分布。IHC证实了视网膜褶皱中的光感受器身份。地图状发育不良斑块不是局灶性脱离，但在cSLO自体荧光模式下具有内部视网膜结构紊乱和强烈的自体荧光，其主要限于地图状病变，结论我们认为地图样病变的自发荧光特征与视网膜内层破裂有关，与血管周围或浸润的巨噬细胞和巨噬细胞的吞噬作用有关。细胞碎片同时，我们建议重新构建检查表，以区分散在分布的皱褶和血管周围分布的皱褶，因为后者可能代表drd的携带者。在后一组中，DNA测试将是提供特定育种建议的有用工具。
Purpose To examine the in vivo microanatomy of retinal folds and geographic lesions in dogs with acquired or inherited retinal dysplasia.Material and methods Thirteen dogs had retinal microanatomy evaluation under general anesthesia using cSLO/sdOCT; two eyes had noninherited multifocal retinal folds, five had inherited multifocal retinal folds (drd1 or drd2), and 10 geographic retinal dysplasia. Retinas from two drd2 carrier dogs were examined by histology and immunohistochemistry (IHC) after in vivo imaging.Results Retinal folds are the common feature of acquired focal/multifocal or geographic retinal dysplasia, are indistinguishable structurally from those associated with syndromic oculoskeletal dysplasia, and represent outer nuclear layer invaginations and rosettes visible by sdOCT. In dogs heterozygous for oculoskeletal dysplasia, the folds form clusters in a perivascular distribution along superior central vessels. IHC confirmed photoreceptor identity in the retinal folds. The geographic dysplasia plaques are not focally detached, but have inner retinal disorganization and intense autofluorescence in cSLO autofluorescence mode that is mainly limited to the geographic lesion, but is not uniform and in some extends beyond the plaques.Conclusion We propose that the autofluorescent characteristic of the geographic lesions is associated with an inner retinal disruption associated with perivascular or infiltrating macrophages and phagocytosis of cellular debris. As well, we suggest restructuring the examination forms to distinguish the folds that are sporadically distributed from those that have a perivascular distribution as the latter likely represent carriers for drd. In this latter group, DNA testing would be a helpful tool to provide specific breeding advice.