PSM2, a novel protein similar to MCAF2, is involved in the mouse embryonic and adult male gonad development
PSM2, a novel protein similar to MCAF2, is involved in the mouse embryonic and adult male gonad development
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DOI：
10.1007/s11033-006-0001-z
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发表时间：
2006-09
影响因子：
2.8
通讯作者：
Huan Cai;Jiarui Hu;P. Song;W. Gong
中科院分区：
文献类型：
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作者：
Huan Cai;Jiarui Hu;P. Song;W. Gong
通过对RIKEN数据库的筛选，我们获得了小鼠卵巢和子宫来源的克隆(RIKEN克隆ID：5031403I04)。多组织表达分析表明，该克隆在卵巢中特异表达，在睾丸中表达水平较高。在小鼠早期胚胎中表达，尤其是在交配后11.5d的胚胎性腺中表达。此外，它还从新生儿睾丸持续表达到成人。用睾丸切片进行原位杂交，发现该基因定位于精原细胞、圆形精子细胞，主要定位于精母细胞。我们从小鼠睾丸中克隆了该基因。该基因由10个外显子组成，大小约48kb，位于小鼠16号染色体上。该基因编码319个氨基酸组成的核蛋白，含有一个卷曲的基序。该蛋白与人MCAF2(含染色质相关因子2)有很高的相似性，因此我们将其命名为PSM2(类似于MCAF2的蛋白)。因此，我们推测PSM2可能通过保守结构域与一些重要的伴侣相互作用，并参与性腺发育过程中的转录调控。
By screening RIKEN database, we obtained a mouse ovary and uterus derived clone (RIKEN clone ID: 5031403I04). Multiple tissues expression analysis revealed that the clone was specifically expressed in ovary and to a higher extent in testis. It is expressed in early mouse embryo, especially in the embryonic gonad from 11.5 dpc (days post coitus). Furthermore, it is also expressed continuously from newborn testis to adult. Using testis sectionsin situ hybridization, we found the mRNA was localized to spermatogonia, round spermatids and mainly to spermatocytes. We cloned the cDNA from mouse testis. The gene consists of 10 exons spanning approximately 48 kb on mouse chromosome 16. The cDNA encodes a putative nuclear protein of 319 amino acids containing a coiled-coil motif. The deduced protein has high similarity with human MCAF2 (MBD1-containing chromatin-associated factor 2), so we termed it as PSM2 (protein similar to MCAF2) in this article. We therefore hypothesized that PSM2 might interact with some important partners by the conserved domain and be involved in the transcription modulation during gonad development.