Nucleic Acid Detection of Plant Genes Using CRISPR-Cas13
Nucleic Acid Detection of Plant Genes Using CRISPR-Cas13
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DOI：
10.1089/crispr.2019.0011
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发表时间：
2019-06-01
期刊：
影响因子：
3.7
通讯作者：
Zhang, Feng
中科院分区：
文献类型：
--
作者：
Abudayyeh, Omar O.;Gootenberg, Jonathan S.;Zhang, Feng
核酸检测在农业应用中至关重要，包括育种过程中的性状检测、害虫监测和病原体鉴定。在这里，我们使用改良版的基于crispr的核酸检测平台SHERLOCK来量化大豆混合物中草甘膦抗性基因的水平，并在一次反应中检测多个植物基因。SHERLOCK具有快速（类似于15分钟）、定量和便携的特点，可以将粗大豆提取物作为输入材料进行最小核酸样品制备。这种现场准备的SHERLOCK平台具有基于颜色的横向流量读数，可用于一系列农业应用中的基因检测和定量。
Nucleic acid detection is vital for agricultural applications including trait detection during breeding, pest surveillance, and pathogen identification. Here, we use a modified version of the CRISPR-based nucleic acid detection platform SHERLOCK to quantify levels of a glyphosate resistance gene in a mixture of soybeans and to detect multiple plant genes in a single reaction. SHERLOCK is rapid (similar to 15 min), quantitative, and portable, and can process crude soybean extracts as input material for minimal nucleic acid sample preparation. This field-ready SHERLOCK platform with color-based lateral flow readout can be applied for detection and quantitation of genes in a range of agricultural applications.