CLONING AND EXPRESSION OF APICAL MEMBRANE WATER CHANNEL OF RAT-KIDNEY COLLECTING TUBULE
CLONING AND EXPRESSION OF APICAL MEMBRANE WATER CHANNEL OF RAT-KIDNEY COLLECTING TUBULE
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DOI：
10.1038/361549a0
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发表时间：
1993-02-11
期刊：
影响因子：
64.8
通讯作者：
SASAKI, S
中科院分区：
文献类型：
--
作者：
FUSHIMI, K;UCHIDA, S;SASAKI, S
对大多数哺乳动物来说，浓缩尿液是防止体内水分流失的必要条件。浓缩尿是在抗利尿素的作用下产生的，通过高透水膜从肾收集小管的管腔中经上皮回收水。在这个肾元段，抗利尿素通过进出顶膜的水通道的内分泌和胞外分泌调节水的渗透性。CHIP28是红细胞和肾近端小管中的一个水通道，但在肾收集小管中不表达。在这里，我们报道了WCH-CD的互补DNA的克隆，WCH-CD是肾集合小管顶端膜的一个水通道。WCH-CD与CHIP28的氨基酸序列相同42%。WCH-CD转录本仅在肾收集小管中检测到。免疫组织化学表明，WCH-CD定位于肾集合小管细胞的顶端区域。WCH-CD在爪蟾卵母细胞中的表达可显著提高其渗透性。WCH-CD的功能表达及局限于肾集小管的顶端区域，提示WCH-CD是抗利尿激素调控的水通道。
CONCENTRATING urine is mandatory for most mammals to prevent water loss from the body. Concentrated urine is produced in response to vasopressin by the transepithelial recovery of water from the lumen of the kidney collecting tubule through highly water-permeable membranes1,2. In this nephron segment, vasopressin regulates water permeability by endo- and exocytosis of water channels from or to the apical membrane3,4. CHIP28 is a water channel in red blood cells and the kidney proximal tubule5, but it is not expressed in the collecting tubule6. Here we report the cloning of the complementary DNA for WCH-CD, a water channel of the apical membrane of the kidney collecting tubule. WCH-CD is 42% identical in amino-acid sequence to CHIP28. WCH-CD transcripts are detected only in the collecting tubule of the kidney. Immunohistochemically, WCH-CD is localized to the apical region of the kidney collecting tubule cells. Expression of WCH-CD in Xenopus oocytes markedly increases osmotic water permeability. The functional expression and the limited localization of WCH-CD to the apical region of the kidney collecting tubule suggest that WCH-CD is the vasopressin-regulated water channel.