DEVELOPMENT OF A SUPERFLUID-HELIUM STAGE FOR HIGH-RESOLUTION ELECTRON-MICROSCOPY
DEVELOPMENT OF A SUPERFLUID-HELIUM STAGE FOR HIGH-RESOLUTION ELECTRON-MICROSCOPY
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DOI：
10.1016/0304-3991(91)90159-4
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发表时间：
1991-12-01
期刊：
影响因子：
2.2
通讯作者：
HARADA, Y
中科院分区：
文献类型：
--
作者：
FUJIYOSHI, Y;MIZUSAKI, T;HARADA, Y
本文报道了一种新型低温高分辨电子显微镜载物台的研制。用超流氦将载物台冷却到1.5K，这种低温显微镜使作者能够在1.5K的载物台温度下记录分辨率为0.26nm的氯化铜酞菁的图像。该低温台的低液氦消耗率使得低温显微镜可以连续运行5 h以上，而无需向低温台槽中再填充液氦和液氮。该平台已通过耐久性测试超过5年。这种电子低温显微镜还配备了一个易于操作的低温转移装置，使冷冻水合样品在低温条件下交换成为可能，而没有任何可观察到的污染。
The development of a new low-temperature stage for high-resolution electron cryo-microscopy is reported here. The stage is cooled down to 1.5 K by superfluid helium, and this cryo-microscope has enabled the authors to record images of chlorinated copper-phthalocyanine to a resolution of 0.26 nm at a stage temperature of 1.5 K. The low consumption rate of liquid helium of this cryo-stage has made possible the continuous operation of the cryo-microscope for more than 5 h without having to refill the cryo-stage tanks with liquid helium and liquid nitrogen. The stage has been tested for durability for more than 5 years. This electron cryo-microscope has also been equipped with an easily operated cryo-transfer device which has made possible the exchange of frozen-hydrated specimens under cryogenic conditions without any observable contamination.