Surface sialic acids taken from the host allow trypanosome survival in Tsetse fly vectors
Surface sialic acids taken from the host allow trypanosome survival in Tsetse fly vectors
复制标题
DOI：
10.1084/jem.20030635
复制
发表时间：
2004-05-17
影响因子：
15.3
通讯作者：
Kinoshita, T
中科院分区：
文献类型：
--
作者：
Nagamune, K;Acosta-Serrano, A;Kinoshita, T
非洲锥虫布氏锥虫（Trypanosoma brucei）通过吸血采采蝇传播，引起人类昏睡病和牲畜Nagana病。在苍蝇的肠道中，锥虫在消化和杀锥虫的环境中存活，增殖并转移到唾液腺中，在那里它们变得感染下一个哺乳动物宿主。在这里，我们表明，在采采蝇的成功生存，锥虫使用转唾液酸酶转移唾液酸，他们不能从主机的糖缀合物合成的糖基磷脂酰肌醇（GPIs），这是大量表达在其表面。由于GPI锚定的转唾液酸酶的产生缺陷而缺乏唾液酸的锥虫不能在肠中存活，但当通过可溶性转唾液酸酶进行唾液酸化时，其恢复了存活的能力。因此，表面唾液酸似乎可以保护寄生虫免受采采蝇中肠消化和杀锥虫环境的影响。
The African trypanosome Trypanosoma brucei, which causes sleeping sickness in humans and Nagana disease in livestock, is spread via blood-sucking Tsetse flies. In the fly's intestine, the trypanosomes survive digestive and trypanocidal environments, proliferate, and translocate into the salivary gland, where they become infectious to the next mammalian host. Here, we show that for successful survival in Tsetse flies, the trypanosomes use trans-sialidase to transfer sialic acids that they cannot synthesize from host's glycoconjugates to the glycosylphosphatidylinositols (GPIs), which are abundantly expressed on their surface. Trypanosomes lacking sialic acids due to a defective generation of GPI-anchored trans-sialidase could not survive in the intestine, but regained the ability to survive when sialylated by means of soluble trans-sialidase. Thus, surface sialic acids appear to protect the parasites from the digestive and trypanocidal environments in the midgut of Tsetse flies.