The Sox-domain containing gene Dichaete/fish-hook acts in concert with vnd and ind to regulate cell fate in the Drosophila neuroectoderm.
The Sox-domain containing gene Dichaete/fish-hook acts in concert with vnd and ind to regulate cell fate in the Drosophila neuroectoderm.
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发表时间：
2002-03
期刊：
影响因子：
4.6
通讯作者：
Guoyan Zhao;J. Skeath
中科院分区：
文献类型：
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作者：
Guoyan Zhao;J. Skeath
在果蝇胚胎中枢神经系统中，神经干细胞，称为神经母细胞，以特定位置的方式获得命运。最近的工作已经确定了一组基因，这些基因沿着背腹轴发挥作用，使在不同背腹区发育的神经母细胞获得不同的命运。这些基因包括进化上保守的转录因子腹神经索缺陷和中间神经母细胞缺陷，以及果蝇EGF受体。我们发现含有SOX结构域的Dichaete/Fish-Hook基因在沿着DV轴形成神经外胚层的过程中也起着至关重要的作用。Dichaete在神经外胚层的内侧和中间柱中表达，突变分析表明Dichaete在这些区域调节细胞命运和神经母细胞的形成。分子上位性试验、双突变分析和剂量敏感相互作用表明，在这些过程中，Dichaete与腹神经索缺陷和中间神经母细胞缺陷平行发挥作用，并在EGF受体信号的下游介导其对发育的影响。这些结果表明Dichaete是神经外胚层中背腹模式的重要调节因子，并表明Dichaete与腹神经索缺陷和中间神经母细胞缺陷共同作用来调节神经外胚层中的模式和细胞命运。
In the Drosophila embryonic central nervous system, neural stem cells, called neuroblasts, acquire fates in a position-specific manner. Recent work has identified a set of genes that functions along the dorsoventral axis to enable neuroblasts that develop in different dorsoventral domains to acquire distinct fates. These genes include the evolutionarily conserved transcription factors ventral nerve cord defective and intermediate neuroblasts defective, as well as the Drosophila EGF receptor. We show that the Sox-domain-containing gene Dichaete/fish-hook also plays a crucial role to pattern the neuroectoderm along the DV axis. Dichaete is expressed in the medial and intermediate columns of the neuroectoderm, and mutant analysis indicates that Dichaete regulates cell fate and neuroblast formation in these domains. Molecular epistasis tests, double mutant analysis and dosage-sensitive interactions demonstrate that during these processes, Dichaete functions in parallel with ventral nerve cord defective and intermediate neuroblasts defective, and downstream of EGF receptor signaling to mediate its effect on development. These results identify Dichaete as an important regulator of dorsoventral pattern in the neuroectoderm, and indicate that Dichaete acts in concert with ventral nerve cord defective and intermediate neuroblasts defective to regulate pattern and cell fate in the neuroectoderm.