Cardiac neural crest is necessary for normal addition of the myocardium to the arterial pole from the secondary heart field.
Cardiac neural crest is necessary for normal addition of the myocardium to the arterial pole from the secondary heart field.
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DOI：
10.1016/j.ydbio.2005.02.011
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发表时间：
2005-05
影响因子：
2.7
通讯作者：
K. Waldo;M. Hutson;H. Stadt;M. Zdanowicz;Jaroslaw Zdanowicz;M. Kirby
中科院分区：
文献类型：
--
作者：
K. Waldo;M. Hutson;H. Stadt;M. Zdanowicz;Jaroslaw Zdanowicz;M. Kirby
在心脏神经嵴消融的胚胎中，次级心脏场不能将心肌细胞添加到流出道，并且管的伸长是缺陷的（Yelbuz，T.M.，Waldo，K.L.，Kumiski，D. H.，施塔特，H.A.，沃尔夫，R. R.，莱瑟伯里湖Kirby，M. L.，2002.神经嵴消融后流出道缩短导致心脏环改变。circ. Res. 106，504 - 510.）。自该研究以来，我们已经表明，次级心脏场向动脉极提供心肌和平滑肌（Waldo，K.L.，赫特森，MR，C.C.沃德Zdanowicz，M.，施塔特，H.A.，Kumiski，D.，Abu-Issa河，Kirby，M. L.，2005.次级心野为发育中心脏的动脉极提供心肌和平滑肌。dev. 281，78 - 90）。本研究旨在确定神经嵴消融后两种细胞类型的添加是否被破坏。标记实验证实，神经嵴消融后心肌成分未能添加到流出道。注定要进入流出心肌的细胞不能迁移，并留在流出心肌与动脉极底部新生平滑肌的交界处。相反，血管平滑肌成分在神经嵴消融后正常添加到动脉极。当心肌不被添加到流出道时，流出连接咽的点不像正常情况下那样向尾部移动，主动脉囊较小并且不能伸长，导致流出道与主动脉弓尾动脉的异常连接。
In cardiac neural-crest-ablated embryos, the secondary heart field fails to add myocardial cells to the outflow tract and elongation of the tube is deficient (Yelbuz, T.M., Waldo, K.L., Kumiski, D.H., Stadt, H.A., Wolfe, R.R., Leatherbury, L., Kirby, M.L., 2002. Shortened outflow tract leads to altered cardiac looping after neural crest ablation. Circ. Res. 106, 504–510.). Since that study, we have shown that the secondary heart field provides both myocardium and smooth muscle to the arterial pole (Waldo, K.L., Hutson, M.R., Ward, C.C., Zdanowicz, M., Stadt, H.A., Kumiski, D., Abu-Issa, R., Kirby, M.L., 2005. Secondary heart field contributes myocardium and smooth muscle to the arterial pole of the developing heart. Dev. Biol. 281, 78–90). The present study was undertaken to determine whether addition of both cell types is disrupted after neural crest ablation. Marking experiments confirm that the myocardial component fails to be added to the outflow tract after neural crest ablation. The cells destined to go into the outflow myocardium fail to migrate and are left at the junction of the outflow myocardium with the nascent smooth muscle at the base of the arterial pole. In contrast, the vascular smooth muscle component is added to the arterial pole normally after neural crest ablation. When the myocardium is not added to the outflow tract, the point where the outflow joins the pharynx does not move caudally as it normally should, the aortic sac is smaller and fails to elongate resulting in abnormal connections of the outflow tract with the caudal aortic arch arteries.