Viral immunology. Comprehensive serological profiling of human populations using a synthetic human virome.
Viral immunology. Comprehensive serological profiling of human populations using a synthetic human virome.
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DOI：
10.1126/science.aaa0698
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发表时间：
2015-06-05
期刊：
影响因子：
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通讯作者：
Elledge SJ
中科院分区：
文献类型：
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作者：
Xu GJ;Kula T;Xu Q;Li MZ;Vernon SD;Ndung'u T;Ruxrungtham K;Sanchez J;Brander C;Chung RT;O'Connor KC;Walker B;Larman HB;Elledge SJ
人类病毒组在健康和免疫中起着重要作用。然而，目前用于检测病毒感染和抗病毒应答的方法具有有限的通量和覆盖范围。在这里，我们提出了VirScan，一种高通量的方法来全面分析抗病毒抗体，使用免疫沉淀和大规模并行DNA测序的噬菌体库显示来自所有人类病毒的蛋白质组范围的肽。我们在四大洲的569人中检测了超过108种抗体-肽相互作用，几乎是先前确定的病毒表位数量的两倍。我们检测到平均每人10种病毒的抗体和至少两个人84种病毒的抗体。虽然特定的病毒暴露率在人群中是异质性的，但抗体反应针对每种病毒的惊人保守的“公共表位”，这表明它们可能会引发高度相似的抗体。VirScan是研究病毒组和免疫系统之间相互作用的强大方法。
The human virome plays important roles in health and immunity. However, current methods for detecting viral infections and antiviral responses have limited throughput and coverage. Here, we present VirScan, a high-throughput method to comprehensively analyze antiviral antibodies using immunoprecipitation and massively parallel DNA sequencing of a bacteriophage library displaying proteome-wide peptides from all human viruses. We assayed over 108 antibody-peptide interactions in 569 humans across four continents, nearly doubling the number of previously established viral epitopes. We detected antibodies to an average of 10 viral species per person and 84 species in at least two individuals. Although rates of specific virus exposure were heterogeneous across populations, antibody responses targeted strikingly conserved “public epitopes” for each virus, suggesting that they may elicit highly similar antibodies. VirScan is a powerful approach for studying interactions between the virome and the immune system.