INDUCTION OF THERMOTOLERANCE AND ENHANCED HEAT-SHOCK PROTEIN-SYNTHESIS IN CHINESE-HAMSTER FIBROBLASTS BY SODIUM ARSENITE AND BY ETHANOL
INDUCTION OF THERMOTOLERANCE AND ENHANCED HEAT-SHOCK PROTEIN-SYNTHESIS IN CHINESE-HAMSTER FIBROBLASTS BY SODIUM ARSENITE AND BY ETHANOL
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DOI：
10.1002/jcp.1041150203
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发表时间：
1983-01-01
影响因子：
5.6
通讯作者：
LI, GC
中科院分区：
文献类型：
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作者：
LI, GC
在细胞中，被称为“热休克”蛋白的蛋白质家族的合成在对各种各样的环境应激的反应中得到增强。这表明这些蛋白质可能具有在应激条件下细胞存活所必需的功能。热休克蛋白的合成和耐热性的发展之间的因果关系将意味着已知诱导热休克蛋白的合成，如亚砷酸钠，也诱导耐热性的代理。相反，已知诱导耐热性的试剂，如乙醇，也会增强热休克蛋白的合成。为了验证这一假设，我研究了亚砷酸钠或乙醇处理对中国仓鼠卵巢HA-1细胞蛋白质合成和细胞存活的影响。无论是亚砷酸钠或乙醇处理后，热休克蛋白的合成大大增强比未经处理的细胞。同时，当暴露于任一试剂的细胞通过随后的热处理进行挑战时，细胞存活率增加多达104倍。热激蛋白的合成与耐热性的形成密切相关。单个蛋白质的定性分析表明，70，000和87，000分子量蛋白质的合成最接近地反映了耐热性的发展。因此，这些结果有力地加强了这样一个假设，即在特定的压力下，热休克蛋白的合成增强和细胞存活之间存在因果关系。
Synthesis of a family of proteins called “heat shock” proteins is enhanced in cells in response to a wide variety of environmental stresses. This suggests that these proteins may have functions essential to cell survival under stressful conditions. A causative relationship between heat shock protein synthesis and development of thermotolerance would imply that agents known to induce heat shock protein synthesis, such as sodium arsenite, also induce thermotolerance. Conversely, agents known to induce thermotolerance, such as ethanol, would also enhance heat shock protein synthesis. To test this hypothesis, I have examined the effect of sodium arsenite or ethanol treatment on protein synthesis and cell survival in Chinese hamster ovary HA‐1 cells. After either sodium arsenite or ethanol treatment, the synthesis of heat shock proteins was greatly enhanced over that of untreated cells. In parallel, cell survival was increased as much as 104‐fold when cells exposed to either agent were challenged by a subsequent heat treatment. The synthesis of heat shock proteins correlated well with the development of thermotolerance. A qualitative analysis of individual proteins suggests that the synthesis of 70,000 and 87,000 molecular weight proteins most closely mirrored the development of thermotolerance. The results, therefore, strongly reinforce the hypothesis that a causal relationship exists between the enhanced synthesis of heat shock protein and cell survival under specific stresses.