Endotoxin stimulates endothelin-release in vivo and in vitro as determined by radioimmunoassay.
Endotoxin stimulates endothelin-release in vivo and in vitro as determined by radioimmunoassay.
复制标题
通过放射免疫测定确定内毒素在体内和体外刺激内皮素释放。
DOI：
10.1016/0006-291x(89)91372-7
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发表时间：
1989
影响因子：
3.1
通讯作者：
Kon,V
中科院分区：
文献类型：
--
作者：
Sugiura,M;Inagami,T;Kon,V
内毒素注射后10-15分钟内，大鼠血清中内皮素免疫反应性明显升高。内皮素水平为117 ± 11.5 pg/ml（平均值± SE，N=4）与对照组中不可检测水平（小于2 pg/ml，N=4）相比。我们还观察到，在0.1和10.0 μ g/ml的内毒素浓度范围内，内毒素从培养的牛转化的胸主动脉内皮细胞释放内皮素的显着刺激。血清对于内毒素的刺激作用是必不可少的，尽管血清本身在所用浓度（1%）下未显示出任何作用。这些结果表明，内皮素在介导内毒素引起的病理生理反应中起重要作用。采用高灵敏度放射免疫法测定内皮素水平。
A marked increase in immunoreactive endothelin was observed in rat serum collected within 10–15 min after infusion of endotoxin. Endothelin level was 117 ± 11.5 pg/ml (mean ± S.E., N=4) in rats exposed to endotoxin as compared with undetectable levels (less than 2 pg/ml, N=4) in controls. We have also observed a significant stimulation of endothelin-release by endotoxin from cultured bovine transformed thoractic aortic endothelial cells at concentrations of endotoxin ranging between 0.1 and 10.0 ug/ml. Serum was indispensable for the stimulating effect of endotoxin, although serum itself did not show any effect at the concentration used (1%). These results suggest that endothelin plays an important role in mediation of pathophysiological responses caused by endotoxin. The levels of endothelin were measured by radioimmunoassay with high sensitivity.