Temporal expression patterns of BMP receptors and collagen II (B) during periosteal chondrogenesis.
Temporal expression patterns of BMP receptors and collagen II (B) during periosteal chondrogenesis.
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骨膜软骨形成过程中 BMP 受体和 II 型胶原 (B) 的时间表达模式。
DOI：
10.1016/s0736-0266(01)00078-x
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
O'Driscoll,ShawnW
中科院分区：
文献类型：
--
作者：
Sanyal,Arunik;Oursler,MerryJo;Clemens,VictoriaR;Fukumoto,Takumi;Fitzsimmons,JamesS;O'Driscoll,ShawnW
Articular cartilage has a limited ability to repair itself. Periosteal grafts have chondrogenic potential and are used clinically to repair defects in articular cartilage. An organ culture model system for in vitro rabbit periosteal chondrogenesis has been established to study the molecular events of periosteal chondrogenesis in vitro. In this model, bone morphogenetic protein‐2 (BMP2) mRNA expression was found to be upregulated in the first 12 h. BMPs usually transduce their signals through a receptor complex that includes type II and either type IA or type IB BMP receptors. Receptors IA and IB play distinct roles during limb development. We have examined the temporal expression patterns for the mRNAs of these receptors using our experimental model. The mRNA expression patterns of these three BMP receptors differed from one another in periosteal explants during chondrogenesis. When these explants were cultured under chondrogenic conditions (agarose suspension with TGF‐β1 added to the media for the first 2 days), the expression of BMPRII mRNA and that of BMPRIA mRNA varied only slightly and persisted over a long time. In contrast, the expression of BMPRIB mRNA was upregulated within 12 h, peaked at day 5, and fell to a level that was barely detected beyond day 21. Moreover, the expression of BMPRIB mRNA preceded that of collagen type IIB mRNAs, a marker for matrix‐depositing chondrocytes. These data support a role for coordinate expression of BMP2 and its receptors early during periosteal chondrogenesis. © 2002 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.
