In Vitro Assay for the Rap GTPase-Activating Protein Activity of the Purified Cytoplasmic Domain of Plexin.
In Vitro Assay for the Rap GTPase-Activating Protein Activity of the Purified Cytoplasmic Domain of Plexin.
复制标题
Plexin 纯化细胞质结构域 Rap GTP 酶激活蛋白活性的体外测定。
DOI：
10.1007/978-1-4939-6448-2_7
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Zhang,Xuewu
中科院分区：
文献类型：
--
作者：
Pascoe,HeathG;Wang,Yuxiao;Zhang,Xuewu
Plexins are cell surface receptors that bind semaphorins and regulate essential processes such as axon guidance and angiogenesis. The cytoplasmic regions of plexins contain a functionally essential GTPase-activating protein (GAP) domain, which initiates downstream signaling by specifically inactivating the Rap GTPase. Here we describe the methods for expression and purification of the plexin cytoplasmic region inE. coli, and characterization of its GAP activity using a photometric assay. We also provide a protocol for measuring GAP activity of single-chain constructs with Rap covalently linked to the plexin cytoplasmic region.
