HISTAMINE AND HISTIDINE-DECARBOXYLASE ARE CORRELATED WITH MUCOSAL REPAIR IN RAT SMALL-INTESTINE AFTER ISCHEMIA-REPERFUSION
HISTAMINE AND HISTIDINE-DECARBOXYLASE ARE CORRELATED WITH MUCOSAL REPAIR IN RAT SMALL-INTESTINE AFTER ISCHEMIA-REPERFUSION
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DOI：
10.1172/jci115552
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发表时间：
1992-01-01
影响因子：
15.9
通讯作者：
TSO, P
中科院分区：
文献类型：
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作者：
FUJIMOTO, K;IMAMURA, I;TSO, P
本实验旨在探讨组胺和组氨酸脱羧酶(HDC)在小肠缺血再灌注(I/R)损伤修复中的作用。结扎肠系膜上动脉15min后再灌流。在空肠黏膜，组胺含量和HDC活性在I/R后增加，肠系膜淋巴组织胺产量在I/R后也增加。HDC活性的增加，粘膜和淋巴组胺水平的增加可被HDC的自杀抑制剂α-氟甲基组氨酸(α-FMH)抑制。α-FMH还可减弱I/R后正常观察到的鸟氨酸脱羧酶(ODC)活性的升高。I/R后24小时，食物中脂质向淋巴的转运显著减少，而I/R后48小时恢复正常。α-FMH抑制剂导致I/R后48小时脂质转运持续不足。这种持续的功能损害与HDC活性和组胺含量对I/R的钝化反应有关。我们的结果表明，组胺和HDC参与了I/R后48小时观察到的粘膜功能的恢复，至少部分与此有关。组胺对ODC活性的刺激作用。
The aim of this experiment was to demonstrate whether histamine and histidine decarboxylase (HDC) contribute to mucosal repair in small intestine subjected to ischemia-reperfusion (I/R). The superior mesenteric artery was occluded for 15 min followed by reperfusion. In jejunal mucosa, histamine content and HDC activity increased after I/R. Histamine output in mesenteric lymph was also elevated after I/R. These increases in HDC activity, and mucosal and lymph histamine levels were suppressed by pretreatment of alpha-fluoromethylhistidine (alpha-FMH), a suicide inhibitor of HDC. Alpha-FMH also attenuated the increase of ornithine decarboxylase (ODC) activity normally observed after I/R. Transport of dietary lipid into lymph markedly decreased at 24 h after I/R, yet it was restored to normal at 48 h after I/R. Alpha-FMH inhibitor led to a sustained deficit in lipid transport at 48 h after I/R. This sustained functional impairment in alpha-FMH treated animals was associated with blunted responses of HDC activity and histamine content to I/R. Our results suggest that histamine and HDC contribute to the restoration in mucosal function observed at 48 h after I/R. This response may be related, at least in part, to stimulation of ODC activity by histamine.