Nuclear Progesterone Receptor Expression in the Human Fetal Membranes and Decidua at Term Before and After Labor
Nuclear Progesterone Receptor Expression in the Human Fetal Membranes and Decidua at Term Before and After Labor
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DOI：
10.1177/1933719108328616
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发表时间：
2009-04-01
影响因子：
2.9
通讯作者：
Mesiano, Sam
中科院分区：
文献类型：
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作者：
Merlino, Amy;Welsh, Toni;Mesiano, Sam
为了探讨孕酮如何影响人类妊娠，我们通过评估核孕酮受体、孕酮受体A和孕酮受体B的表达和定位程度，确定了足月时胎膜（羊膜、绒毛膜和蜕膜）内的孕酮靶细胞。在分娩开始前（n = 7）和分娩开始后（n = 7）进行足月剖宫产后获得胎膜（分为羊膜和绒毛膜-蜕膜）。核孕激素受体的表达测定核孕激素受体mRNA（定量逆转录-聚合酶链反应）和蛋白质（蛋白质印迹法）的丰度。通过免疫组织化学确定nPR的定位。孕激素受体-A和孕激素受体-B的mRNA和蛋白质水平最高的绒毛膜-蜕膜，并没有改变与劳动。核孕酮受体mRNA和蛋白在羊膜中几乎检测不到。核孕酮受体免疫染色仅在蜕膜细胞核中检测到。这些结果表明，蜕膜，而不是羊膜和绒毛膜，是一个直接的目标核孕酮受体介导的孕酮行动在人类怀孕期间。
To explore how progesterone affects human pregnancy, we identified the progesterone target cells within the fetal membranes (amnion, chorion, and decidua) at term by assessing the extent of expression and localization of the nuclear progesterone receptors, progesterone receptor-A and progesterone receptor-B. Fetal membranes (separated into amnion and chorion-decidua) were obtained after term cesarean deliveries performed before (n = 7) and after (n = 7) labor onset. Nuclear progesterone receptor expression was determined by the abundance of nuclear progesterone receptor mRNAs (by quantitative reverse transcriptase-polymerase chain reaction) and proteins (by western blotting). Localization of nPRs was determined by immunohistochemistry. Progesterone receptor-A and progesterone receptor-B mRNA and protein levels were highest in the chorion-decidua and did not change in association with labor. Nuclear progesterone receptor mRNAs and proteins were barely detectable in amnion. Nuclear progesterone receptor immunostaining was detected only in the nucleus of decidual cells. These findings suggest that the decidua, and not the amnion and chorion, is a direct target for nuclear progesterone receptor-mediated progesterone actions during human pregnancy.