A Simple, Lightweight, and Low-Cost Customizable Multielectrode Array for Local Field Potential Recordings.
A Simple, Lightweight, and Low-Cost Customizable Multielectrode Array for Local Field Potential Recordings.
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DOI：
10.1523/eneuro.0212-23.2023
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发表时间：
2023-08
期刊：
影响因子：
3.4
通讯作者：
Khokhar, Jibran Y.
中科院分区：
文献类型：
--
作者：
Amissah, Richard Quansah;Albeely, Abdalla M.;Bragg, Elise M.;Perreault, Melissa L.;Doucette, Wilder T.;Khokhar, Jibran Y.
局部场电位（LFP）记录是评估大脑系统通信的一种有价值的方法。已经开发了多种方法来收集LFP数据以研究大脑的节律活动。这些方法的范围从使用单个或成束的金属电极到可以针对多个大脑区域的电极阵列。虽然这些电极在收集LFP活动方面是有效的，但它们可能是昂贵的，难以构建的，并且不太适合不同的应用，这可能包括同时靶向多个大脑区域。在这里，一个16通道的可定制的多电极阵列（CMEA），可用于收集LFP数据，同时在大鼠不同的大脑区域的建设过程进行了描述。这些CMEA电极阵列重量轻（<1 g），构建时间短（<1 h），价格实惠（15加元）。CMEA也可以被修改为使用有线和无线神经数据采集系统记录除了LFP活动之外的单单元和多单元活动。此外，这些CMEA可用于在初步研究中探索神经活动（LFP和单单位/多单位活动），然后再购买更昂贵的电极进行针对性研究。总之，这些特性使得所描述的CMEA成为市售多电极阵列的有竞争力的替代方案，因为其简单、低成本和在自由行为的动物中收集LFP数据的效率。
Local field potential (LFP) recording is a valuable method for assessing brain systems communication. Multiple methods have been developed to collect LFP data to study the rhythmic activity of the brain. These methods range from the use of single or bundled metal electrodes to electrode arrays that can target multiple brain regions. Although these electrodes are efficient in collecting LFP activity, they can be expensive, difficult to build, and less adaptable to different applications, which may include targeting multiple brain regions simultaneously. Here, the building process for a 16-channel customizable multielectrode array (CMEA) that can be used to collect LFP data from different brain regions simultaneously in rats is described. These CMEA electrode arrays are lightweight (<1 g), take little time to build (<1 h), and are affordable ($15 Canadian). The CMEA can also be modified to record single-unit and multiunit activity in addition to LFP activity using both wired and wireless neural data acquisition systems. Moreover, these CMEAs can be used to explore neural activity (LFP and single-unit/multiunit activity) in preliminary studies, before purchasing more expensive electrodes for targeted studies. Together, these characteristics make the described CMEA a competitive alternative to the commercially available multielectrode arrays for its simplicity, low cost, and efficiency in collecting LFP data in freely behaving animals.