Detection of carbapenemase activities of Bacteroides fragilis strains with matrix-assisted laser desorption ionization - Time of flight mass spectrometry (MALDI-TOF MS)
Detection of carbapenemase activities of Bacteroides fragilis strains with matrix-assisted laser desorption ionization - Time of flight mass spectrometry (MALDI-TOF MS)
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DOI：
10.1016/j.anaerobe.2014.01.006
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发表时间：
2014-04-01
期刊：
影响因子：
2.3
通讯作者：
Soki, Jozsef
中科院分区：
文献类型：
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作者：
Johansson, Asa;Nagy, Elisabeth;Soki, Jozsef
今天，对碳青霉烯类的耐药性被认为是脆弱拟杆菌中的一个新问题。需氧菌产生的碳青霉烯酶活性已通过观察碳青霉烯类的水解与MALDI-TOF MS检测，但该技术从未用于厌氧菌。我们已经制定了一项检测和验证B中碳青霉烯酶生产的方案。2.5h内出现脆裂。28株B。fragilis进行了测试。在16株cfiA阳性菌株中，所有菌株均显示厄他培南水解，而12株cfiA阴性菌株未显示水解。在所有cfiA阳性菌株中，2，6-吡啶羧酸（DPA）均可抑制厄他培南水解，证实存在金属β-内酰胺酶。在这里，我们展示了一种快速检测B碳青霉烯酶活性的方法。fragilis菌株。(C)2014爱思唯尔有限公司版权所有。
Today resistance against carbapenems is considered an emerging problem in Bacteroides fragilis. Carbapenemase activities produced by aerobic bacteria have been detected by looking at hydrolysis of carbapenems with MALDI-TOF MS, but this technique was never used for anaerobic bacteria. We have developed a protocol for detection and verification of carbapenemase production in B. fragilis within 2.5 h. Twenty-eight strains of B. fragilis were tested. Of the sixteen cfiA-positive strains all showed hydrolysis of ertapenem, whereas the twelve cfiA-negative strains showed no hydrolysis. Ertapenem hydrolysis could be inhibited with 2,6-Pyridinecarboxylic acid (DPA) in all cfiA-positive strains, verifying the presence of the metallo-beta-lactamase. Here we show a rapid way to detect carbapenemase activities of B. fragilis strains. (C) 2014 Elsevier Ltd. All rights reserved.