Integrated Proteome and Phosphoproteome Analyses Reveal Early- and Late-Stage Protein Networks of Traumatic Brain Injury
Integrated Proteome and Phosphoproteome Analyses Reveal Early- and Late-Stage Protein Networks of Traumatic Brain Injury
复制标题
综合蛋白质组和磷酸化蛋白质组分析揭示了创伤性脑损伤的早期和晚期蛋白质网络
DOI：
10.1007/s12031-021-01949-w
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发表时间：
2022-01-12
影响因子：
3.1
通讯作者：
Jiang, Xiaofan
中科院分区：
文献类型：
--
作者：
Huang, Yutao;Zhang, Haofuzi;Jiang, Xiaofan
创伤性脑损伤（TBI）是全世界主要的公共卫生问题。越来越多的证据表明，脑损伤后的病理过程不断演变。在这里，我们确定了差异表达的蛋白质（DEPs）和差异表达的磷蛋白（DEPPs）在早期和晚期阶段的TBI小鼠使用TMT标记，富集的Phos亲和随后，和高分辨率LC-MS/MS分析。随后，进行了综合分析，包括基于功能富集的聚类分析、基序分析、串扰途径/过程富集分析和蛋白质-蛋白质相互作用富集分析，以进一步鉴定早期和晚期的不同和相似的病理生理机制。我们的工作揭示了TBI早期和晚期蛋白质网络的图谱，这为TBI及其后遗症的有用生物标志物和潜在机制提供了线索。
Traumatic brain injury (TBI) is a major public health concern all around the world. Accumulating evidence suggests that pathological processes after brain injury continuously evolve. Here, we identified the differentially expressed proteins (DEPs) and differentially expressed phosphoproteins (DEPPs) in the early and late stages of TBI in mice using TMT labeling, enrichment of Phos affinity followed, and high-resolution LC-MS/MS analysis. Subsequently, integrative analyses, including functional enrichment-based clustering analysis, motif analysis, cross-talk pathway/process enrichment analysis, and protein-protein interaction enrichment analysis were performed to further identify the different and similar pathophysiologic mechanisms in the early and late stage. Our work reveals a map of early and late-stage protein networks in TBI, which shed light on useful biomarkers and the underlying mechanisms in TBI and its sequelae.