Magnetic Stimulation of Dissociated Cortical Neurons on a Planar Mulitelectrode Array*
Magnetic Stimulation of Dissociated Cortical Neurons on a Planar Mulitelectrode Array*
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平面多电极阵列上分离的皮质神经元的磁刺激*
DOI：
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发表时间：
2019
期刊：
影响因子：
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通讯作者：
P. Bhatti
中科院分区：
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作者：
S. Mukesh;Riley Zeller;R. Butera;P. Bhatti
我们进行实验来研究不同刺激参数下磁刺激引起的分离皮质神经元神经活动的变化。进行这些研究的目的是建立在我们之前的工作结果的基础上，这些结果表明磁刺激可能会改善人工耳蜗的性能。使用微型线圈组装磁刺激器。为了检测活动的微小变化，我们使用玻璃基底 MEA 来测量培养范围内突触介导的刺激反应，而不是单个神经元的直接激活。我们的初步研究结果表明，磁刺激与网络范围内的放电率的变化有关，超出了活动自发漂移所预期的范围。这表明我们使用的磁刺激参数能够引起神经活动。然而，我们观察到不同文化和不同刺激参数下神经活动引起的变化类型存在显着差异，一些显示活动增加，另一些显示活动减少。这可能是由于不同实验中刺激激活的神经元（抑制性或兴奋性）的数量和类型存在差异，而这又可能受到刺激器位置和排列的差异、刺激脉冲波形和幅度的差异、或培养密度或细胞形态的差异的影响。我们还将这种刺激技术与电刺激的功耗和加热进行了比较。最后，确定需要优化实验设置以允许更长的实验，以得出明确的结论。
We perform experiments to study the magnetic stimulus-induced changes in neural activity in dissociated cortical neurons with different stimulation parameters. The goal of performing these studies is to build on the results from our previous work that suggested magnetic stimulation may lead to improved performance of cochlear implants. A magnetic stimulator is assembled using a micro-scale coil. To detect small changes in activity, we use glass substrate MEAs to measure culture-wide synaptically-mediated response to stimulation, rather than the direct activation of individual neurons. Our initial findings show magnetic stimulation is associated with changes in network-wide firing rates, beyond those expected by spontaneous drift in activity. This suggests that the magnetic stimulation parameters we used were able to evoke neural activity. However, we observe substantial differences in the type of change induced in neural activity in different cultures and with different stimulation parameters, some showing increases in activity and others showing decreases in activity. This may be due to differences in the number and type of neurons (inhibitory or excitatory) activated by stimulation in different experiments, which in turn may be affected by differences in stimulator location and alignment, differences in stimulus pulse waveform and amplitudes, or differences in culture density or cell morphology. We also compare the power consumption and heating of this stimulation technique with that of electrical stimulation. Finally, a need to optimize the experimental setup to allow longer experiments is identified, to reach definite conclusions.