Analysis of the petunia MADS-box transcription factor family
Analysis of the petunia MADS-box transcription factor family
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DOI：
10.1007/s00438-002-0781-3
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发表时间：
2003-02-01
影响因子：
3.1
通讯作者：
Angenent, GC
中科院分区：
文献类型：
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作者：
Immink, RGH;Ferrario, S;Angenent, GC
转录因子是植物发育的关键调控因子。MADS-box家族是重要的转录因子家族之一，其中至少有80个成员在拟南芥基因组中编码。本研究对矮牵牛MADS-box转录因子家族的23个成员进行了Northern杂交、系统发育和酵母双杂交分析。许多被描述的基因似乎有一个或多个近亲拥有相似的表达模式。对这些蛋白质结合作用的比较表明，一些蛋白质表现出相似的相互作用模式，因此很可能是功能冗余的。从进化的角度来看，他们的编码基因可能来自最近的一次复制事件。此外，蛋白质-蛋白质相互作用模式，结合表达模式和系统发育分类，似乎为鉴定功能同源物提供了良好的标准。根据矮牵牛和拟南芥的这些数据的比较，可以预测这两个物种中几个MADS-box转录因子的功能。
Transcription factors are key regulators of plant development. One of the major groups of transcription factors is the MADS-box family, of which at least 80 members are encoded in the Arabidopsis genome. In this study, 23 members of the petunia MADS-box transcription factor family were investigated by Northern hybridisation, phylogenetic and yeast two-hybrid analyses. Many of the genes characterised appeared to have one or more close relatives that shared similar expression patterns. Comparison of the binding interactions of these proteins revealed that some show similar interaction patterns, and hence are likely to be functionally redundant. From an evolutionary point of view, their coding genes are probably derived from a recent duplication event. Furthermore, protein-protein interaction patterns, in combination with expression patterns and phylogenetic classification, appear to offer good criteria for the identification of functional homologues. Based on comparison of such data between petunia and Arabidopsis, functions can be predicted for several MADS-box transcription factors in both species.