Analysis of type 2 immunity in vivo with a bicistronic IL-4 reporter
Analysis of type 2 immunity in vivo with a bicistronic IL-4 reporter
复制标题
DOI：
10.1016/s1074-7613(01)00186-8
复制
发表时间：
2001-08-01
期刊：
影响因子：
32.4
通讯作者：
Locksley, RM
中科院分区：
文献类型：
--
作者：
Mohrs, M;Shinkai, K;Locksley, RM
效应性T细胞介导获得性免疫和免疫病理学，但体内追踪此类细胞的方法有限。我们设计了一种通过病毒IRES元件与增强型绿色荧光蛋白(EGFP)连接的表达IL-4的敲门小鼠。Th2条件下激活的报告T细胞表现出灵敏而忠实的EGFP表达，并维持内源性IL-4。在尼波氏杆菌感染后，报告表达证实了2型免疫从组织淋巴细胞进化到淋巴结CD4(+)T细胞，后者随后迁移到组织中。EGFP(+)CD4(+)T细胞在组织中的出现，而不是在淋巴结中的出现，是STAT6依赖的。从感染动物身上转移的EGFP(+)CD4(+)T细胞对免疫缺陷小鼠具有保护作用。这些小鼠将为体内免疫评估提供有价值的试剂。
Effector T cells mediate adaptive immunity and immunopathology, but methods for tracking such cells in vivo are limited. We engineered knockin mice expressing IL-4 linked via a viral IRES element with enhanced green fluorescent protein (EGFP). Reporter T cells primed under Th2 conditions showed sensitive and faithful EGFP expression and maintained endogenous IL-4. After Nippostrongylus infection, reporter expression demonstrated the evolution of type 2 immunity from tissue lymphocytes and thence to lymph node CD4(+) T cells, which subsequently migrated into tissue. The appearance of EGFP(+) CD4(+) T cells in tissue, but not in lymph nodes, was Stat6-dependent. Transferred EGFP(+) CD4(+) T cells from infected animals conferred protection against Nippostrongylus to immunodeficient mice. These mice will provide a valuable reagent for assessing immunity in vivo.