Detection of De Novo Dividing Stem Cells In Situ through Double Nucleotide Analogue Labeling.
Detection of De Novo Dividing Stem Cells In Situ through Double Nucleotide Analogue Labeling.
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组织特异性体干细胞的特征在于它们能够驻留在延长的可逆细胞周期停滞状态中，称为静止。维持细胞静止和分裂之间的平衡对于细胞水平的组织稳态至关重要，并且受到许多外在和内在因素的动态调节。由于缺乏直接检测从头分裂细胞的方法，对静止干细胞活化的分析一直具有挑战性。在这里，我们提出并实验验证了一种新的方法，基于双标记与胸苷类似物原位检测从头分裂干细胞。在该方法的概念证明中，我们发现美金刚胺，一种广泛用于阿尔茨海默病治疗的药物和已知的成年海马神经发生的强诱导剂，增加了静止放射状胶质样干细胞分裂周期的招募-海马中成年神经元的主要前体。我们的方法可以应用于评估衰老，病理或药物治疗对发育和成人组织中干细胞隔室中静止干细胞的影响。
Tissue-specific somatic stem cells are characterized by their ability to reside in a state of prolonged reversible cell cycle arrest, referred to as quiescence. Maintenance of a balance between cell quiescence and division is critical for tissue homeostasis at the cellular level and is dynamically regulated by numerous extrinsic and intrinsic factors. Analysis of the activation of quiescent stem cells has been challenging because of a lack of methods for direct detection of de novo dividing cells. Here, we present and experimentally verify a novel method based on double labeling with thymidine analogues to detect de novo dividing stem cells in situ. In a proof of concept for the method, we show that memantine, a drug widely used for Alzheimer’s disease therapy and a known strong inducer of adult hippocampal neurogenesis, increases the recruitment into the division cycle of quiescent radial glia-like stem cells—primary precursors of the adult-born neurons in the hippocampus. Our method could be applied to assess the effects of aging, pathology, or drug treatments on the quiescent stem cells in stem cell compartments in developing and adult tissues.
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DOI：
10.1242/dev.100842
发表时间：
2014-04
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Chakkalakal JV;Christensen J;Xiang W;Tierney MT;Boscolo FS;Sacco A;Brack AS
通讯作者：
Brack AS
DOI：
10.4049/jimmunol.1501162
发表时间：
2016-10-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Koning JJ;Konijn T;Lakeman KA;O'Toole T;Kenswil KJ;Raaijmakers MH;Michurina TV;Enikolopov G;Mebius RE
通讯作者：
Mebius RE
影响因子：
2.5
作者：
Kronenberg, G;Reuter, K;Kempermann, G
通讯作者：
Kempermann, G
DOI：
10.1038/nrm3591
发表时间：
2013-06
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
--
影响因子：
23.9
作者：
Encinas, Juan M.;Michurina, Tatyana V.;Peunova, Natalia;Park, June-Hee;Tordo, Julie;Peterson, Daniel A.;Fishell, Gord;Koulakov, Alex;Enikolopov, Grigori
通讯作者：
Enikolopov, Grigori