In-situ assembly of biocompatible core-shell hierarchical nanostructures sensitized immunosensor for microcystin-LR detection
In-situ assembly of biocompatible core-shell hierarchical nanostructures sensitized immunosensor for microcystin-LR detection
复制标题
用于微囊藻毒素-LR检测的生物相容性核壳分层纳米结构敏化免疫传感器的原位组装
DOI：
10.1016/j.bios.2015.11.072
复制
发表时间：
2016-04-15
影响因子：
12.6
通讯作者：
Liu, Yingju
中科院分区：
文献类型：
--
作者：
Gan, Cuifen;Ling, Li;Liu, Yingju
微囊藻毒素-LR(MC-LR)是一种能引起肝脏功能和结构紊乱、在水生生物体内蓄积并向高级营养层次转移的肝毒素，构建了一种生物相容性的电化学免疫传感器，可以灵敏、选择性地检测MC-LR。制备了三维绒毛状碳纳米管/硅酸钴(CNT@Co Silate)核壳纳米复合材料，并首次将其作为底物固定MC-LR(Ag)抗原，同时制备了Fe3O4纳米团簇/多巴胺/金纳米粒子(Fe3O4@PDA-Au)核壳磁性纳米复合材料作为免疫传感器的标记载体，将第二抗体(Ab2)与辣根过氧化物酶(HRP)偶联。由于纳米材料的毒性在构建包括抗原或抗体的固定化在内的生物传感器中是重要的，因此通过监测与Hela细胞培养后的细胞活力来研究这种纳米复合材料的生物相容性。由于良好的生物相容性，该免疫传感器可以利用碳纳米管@Co硅酸盐中较大的三维绒毛状结构来固定更多的抗原，并通过Fe3O4@PDA-Au标记AB2和HRP来提供高电化学信号。通过对生物分子与纳米材料结合能力的考察和竞争免疫分析条件的优化，该免疫传感器与MC-LR在501.1 mU/L~0.005 mU/L范围内呈良好的线性关系，检出限为0.004 mU/L，且具有良好的特异性、重现性和稳定性，在环境监测中具有潜在的应用前景。(C)2015爱思唯尔B.V.保留所有权利。
Microcystin-LR (MC-LR) is a kind of hepatotoxin which can cause functional and structural disturbances of the liver, accumulate in aquatic organisms and transfer to higher trophic levels, a biocompatible electrochemical immunosensor was constructed to detect MC-LR sensitively and selectively. The three-dimensional villiform-like carbon nanotube/cobalt silicate (CNT@Co silicate) core-shell nanocomposites were synthesized and firstly used as the substrate to immobilize the antigen of MC-LR (Ag), while Fe3O4 nanoclusters/polydopamine/gold nanoparticles (Fe3O4@PDA-Au) core-shell magnetic nanocomposites were prepared as the label carrier of the immunosensor to conjugate the second antibody (Ab(2)) and horse radish peroxidase (HRP). Since the toxicity of nanomaterials is important in the construction of biosensors including the immobilization of antigen or antibody, the biocompatibility of such nano composites were investigated by monitoring the cell viability after culturing with Hela cells. Due to the excellent biocompatibility, the immunosensor can immobilize more antigens by the large surface area of the three-dimensional villiform-like structure in CNT@Co silicate, and provide high electrochemical signals by Fe3O4@PDA-Au labeled Ab2 and HRP. After investigation of the binding capability of biomolecules on nanomaterials and optimization of the conditions in the competitive immunoassay, the proposed electrochemical immunosensor shows a linear response to MC-LR in the range from 0.005 mu/L. to 501.1 mu/L with a detection limit of 0.004 mu/L In addition, the specificity, reproducibility and stability of the immunosensor were also proved to be acceptable, indicating its potential application in environmental monitoring. (C) 2015 Elsevier B.V. All rights reserved.