Anterior epidermis-specific expression of the cuticle gene EDG84A is controlled by many cis-regulatory elements in Drosophila melanogaster
Anterior epidermis-specific expression of the cuticle gene EDG84A is controlled by many cis-regulatory elements in Drosophila melanogaster
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DOI：
10.1007/s00427-005-0013-z
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发表时间：
2005-07
影响因子：
2.4
通讯作者：
Y. Kayashima;S. Hirose;H. Ueda
中科院分区：
文献类型：
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作者：
Y. Kayashima;S. Hirose;H. Ueda
在昆虫变态过程中，蜕皮激素的脉冲会根据身体的不同部位引起许多不同的形态变化。在果蝇中，尽管许多转录因子在蜕皮激素脉冲的作用下以阶段特异性的方式表达，但对变态过程中空间特异性基因表达的调控机制知之甚少。编码蛹角质层蛋白的EDG 84 A基因是蜕皮激素诱导转录因子βFTZ-F1的靶基因之一，在蛹前中期至后期仅在前表皮表达，而βFTZ-F1几乎在所有组织中表达。为了阐明EDG 84 A基因的组织特异性表达的调控机制，我们建立了携带该基因的不同上游区域与LacZ基因融合的转基因果蝇品系，并检测了报告基因的表达模式。转基因果蝇报告基因分析的结果表明，空间特异性表达由至少4个阳性和2个阴性元件控制的转录起始位点附近的263-bp区域内，其中至少有三个显示出空间特异性效应的前体干。这些结果表明，高表达水平和差异表达是通过许多顺式调控元件实现的。
During insect metamorphosis, a pulse of ecdysteroids induces many different morphological changes depending on different parts of the body. InDrosophila, although a number of transcription factors are expressed in a stage-specific manner in response to an ecdysteroid pulse, little is known on the regulatory mechanism for space-specific gene expression during metamorphosis. TheEDG84Agene encoding pupal cuticle protein is one of the targets of ecdysteroid-inducible transcription factor βFTZ-F1 and is expressed only in anterior epidermis of the body during mid- to late prepupal period, whereas βFTZ-F1 is expressed in almost all tissues. To address the regulatory mechanism of the tissue-specific expression of theEDG84Agene, we established transgenic fly lines which carry various upstream regions of the gene fused to theLacZgene and examined the expression pattern of the reporter gene. Results of the transgenic fly reporter assays showed that the space-specific expression is controlled by at least four positive and two negative elements within a 263-bp region near the transcription start site, and at least three of them showed space-specific effects to the anterior body trunk. These results suggest that both high expression level and differential expression are achieved through manycis-regulatory elements.