Does Prohibitin Expression Regulate Sperm Mitochondrial Membrane Potential, Sperm Motility, and Male Fertility?
Does Prohibitin Expression Regulate Sperm Mitochondrial Membrane Potential, Sperm Motility, and Male Fertility?
复制标题
抑制素表达是否调节精子线粒体膜电位、精子活力和男性生育能力？
DOI：
10.1089/ars.2012.4514
复制
发表时间：
2012-08-01
影响因子：
6.6
通讯作者：
Chen, Hong
中科院分区：
文献类型：
--
作者：
Wang, Mei-Jiao;Ou, Jia-Xian;Chen, Hong
抑制素（Prohibitin，PHB）是一种高度保守的精子线粒体膜蛋白，其在体细胞中的缺失与线粒体膜去极化和活性氧（reactive oxygen species，ROS）产生增加有关。我们最近的研究结果表明，人类精液中高水平的氧化剂可能导致男性不育，精子活力可能是氧化损伤的最早和最敏感的指标。基于PHB在线粒体亚区室化和呼吸链组装中的作用，我们研究了精子活动力差（弱精子症，A）和/或精子浓度低（少弱精子症，OA）的不育男性精子的PHB表达和线粒体膜电位（MITO）。在这里，我们证明了MITO是显着降低精子从A和OA科目比正常精子（N）科目，OA比A科目的下降更严重。A和OA受试者精子中的PHB表达也显著较低。在正常精子、弱精子和少弱精子受试者中，发现PHB表达、MITO和精子活力之间存在显著正相关。总的来说，我们的观察结果导致假设，在不育男性的精子质量的一个指标，在MITO和增加ROS水平的改变，它调节精子活力。抗氧化剂。氧化还原信号。17，513-519。
Prohibitin (PHB) is a highly conserved major sperm mitochondrial membrane protein whose absence in somatic cells is associated with mitochondrial membrane depolarization and increased generation of reactive oxygen species (ROS). Our recent findings suggest that high levels of oxidants in human semen may contribute to male infertility and that sperm motility could be the earliest and most sensitive indicator of oxidative damage. Based on PHB's roles in mitochondrial sub-compartmentalization and respiratory chain assembly, we examine sperm PHB expression and mitochondrial membrane potential (MITO) in infertile men with poor sperm motility (asthenospermia, A) and/or low sperm concentrations (oligoasthenospermia, OA). Here, we demonstrate that MITO is significantly lower in sperm from A and OA subjects than in normospermic (N) subjects; the decrease is more severe for OA than for A subjects. PHB expression is also significantly lower in sperm from A and OA subjects. Significantly positive correlations are found among PHB expression, MITO, and sperm motility in normospermic, asthenospermic, and oligoasthenospermic subjects. Collectively, our observations lead to the hypothesis that PHB expression is an indicator of sperm quality in infertile men, and that it regulates sperm motility via an alteration in MITO and increased ROS levels. Antioxid. Redox Signal. 17, 513-519.