Effects of prenatal X-irradiation on the 14th-18th days of gestation on postnatal growth and development in the rat.
Effects of prenatal X-irradiation on the 14th-18th days of gestation on postnatal growth and development in the rat.
复制标题
孕14~18天产前X线照射对大鼠产后生长发育的影响
DOI：
10.1002/tera.1420380506
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Brent,RL
中科院分区：
文献类型：
--
作者：
Jensh,RP;Brent,RL
39只成年Wistar妊娠大鼠被随机分为三个暴露组：0、0.75或1.50 Gy X射线总暴露。从妊娠第14天至第18天，分别以每天0、0.15或0.30戈瑞的剂量照射动物。足月处死大鼠15只，完成形态学分析。24只老鼠被允许生产它们的后代。在出生后的第一天，每窝减少到最多8只幼崽，尽可能保持雄性和雌性后代的数量相等。对187只幼鼠进行了5项反射（空气翻正、体表翻正、视觉放置、负地向性、听觉惊吓）的获得年龄和4项生理指标（耳廓脱离、睁眼、阴道口、睾丸下降）的外观观察。在整个86天的产后期间，足月胎儿和后代的体重都有显著的剂量相关的减轻。出生后生长率（g /day）未受影响。成年子代脑、性腺重量和脏器重量与体质量之比降低。使用PAC50方法，剂量相关的改变发生在几种反射的获得中。所有生理指标均表现出剂量相关的外观延迟。这些结果表明，大鼠在胎儿时期的X辐射暴露会导致出生后生长和生理发育的剂量依赖性改变。这些研究对我们了解妊娠后期产前暴露于电离辐射的长期影响具有重要意义。
Thirty‐nine pregnant adult Wistar strain rats were randomly assigned to one of three exposure groups: 0, 0.75, or 1.50 Gy X‐radiation total exposure. Animals were exposed from the 14th to the 18th days of gestation at 0, 0.15, or 0.30 Gy per day. At term, 15 rats were killed and morphologic analyses were completed. Twenty‐four rats were allowed to deliver their offspring. On the first day of postnatal life, litters were reduced to a maximum of eight pups per litter, with equal numbers of male and female offspring wherever possible. A total of 187 pups were observed for the age of acquisition of five reflexes (air righting, surface righting, visual placing, negative geotaxis, auditory startle) and the appearance of four physiologic markers (pinna detachment, eye opening, vaginal opening, testes descent). There was significant dose‐related weight reduction in term fetuses and offspring throughout the 86‐day postnatal period. Postnatal growth rate (g gained/day) was unaffected. Adult offspring brain and gonadal weight and organ weight:body weight ratios were reduced. Using the PAC50 methodology, dose‐related alterations occurred in the acquisition of several reflexes. All physiologic markers exhibited a dose‐related delay in appearance. These results indicate that fractionated exposure to X‐radiation during the fetal period in the rat results in dose‐dependent alterations in postnatal growth and physiologic development. These studies are important for our understanding of the long‐range effects of prenatal exposure to ionizing radiation late in gestation.