GENETIC FINGERPRINTING OF PIGEONPEA [CAJANUS-CAJAN (L) MILLSP] AND ITS WILD RELATIVES USING RAPD MARKERS
GENETIC FINGERPRINTING OF PIGEONPEA [CAJANUS-CAJAN (L) MILLSP] AND ITS WILD RELATIVES USING RAPD MARKERS
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DOI：
10.1007/bf00223897
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发表时间：
1995-11-01
影响因子：
5.4
通讯作者：
RANJEKAR, PK
中科院分区：
文献类型：
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作者：
RATNAPARKHE, MB;GUPTA, VS;RANJEKAR, PK
随机扩增多态性DNA（RAPD）标记被用于鉴定木豆[Cajanus cajan (L.) Millsp.]栽培品种及其相关野生种。使用任意核苷酸序列的单一引物导致了对各个材料独特的DNA片段的选择性扩增。野生种之间的多态性水平极高，而在木豆栽培品种内检测到的多态性很少。借助不同的引物，所有被研究的栽培品种和野生种都能很容易被区分，这表明RAPD在木豆遗传指纹分析中具有巨大潜力。根据我们的数据，可以确定木豆栽培品种与其野生种之间的遗传关系。
Randomly amplified polymorphic DNA (RAPD) markers were used for the identification of pigeonpea [Cajanus cajan (L.) Millsp.] cultivars and their related wild species. The use of single primers of arbitrary nucleotide sequence resulted in the selective amplification of DNA fragments that were unique to individual accessions. The level of polymorphism among the wild species was extremely high, while little polymorphism was detected within Cajanus cajan accessions. All of the cultivars and wild species under study could be easily distinguished with the help of different primers, thereby indicating the immense potential of RAPD in the genetic fingerprinting of pigeonpea. On the basis of our data the genetic relationship between pigeonpea cultivars and its wild species could be established.