ADIPOSE-TISSUE OF DROSOPHILA-MELANOGASTER .7. DISTRIBUTION OF NUCLEAR-DNA AMOUNTS ALONG THE ANTERIOR POSTERIOR AXIS IN THE LARVAL FAT-BODY
ADIPOSE-TISSUE OF DROSOPHILA-MELANOGASTER .7. DISTRIBUTION OF NUCLEAR-DNA AMOUNTS ALONG THE ANTERIOR POSTERIOR AXIS IN THE LARVAL FAT-BODY
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DOI：
10.1002/jez.1402390110
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发表时间：
1986-07-01
影响因子：
--
通讯作者：
RASCH, EM
中科院分区：
文献类型：
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作者：
BUTTERWORTH, FM;RASCH, EM
在整个幼虫发育过程中，脂肪体细胞核的DNA含量通过细胞光度法测量为沿组织的前-后（A-P）轴沿着位置的函数。在此期间的前3天，每个细胞核的平均DNA量急剧增加了55倍，但在最后一天没有进一步增加。然而，增长率具有区域特异性。每个细胞核的DNA量与其沿组织A-P轴的沿着位置显著相关：向后方向的细胞核含有逐渐增加的DNA量。最小的前核和最大的后核之间的差异高达7倍。此外，对于三个年龄段的研究有一个次梯度在后部区域的斜率是相当陡峭的整体组织梯度。该组织有三个特征性的形态区域，前，中，后，这可以在发育早期识别，并保持整个幼虫期。在发育的最后一天之前，在幼虫生命的最后2天确定的每个区域中细胞的核DNA类别的分布变得固定。DNA梯度的蛋白质存储梯度的意义进行了讨论。
The DNA content of fat body nuclei was measured cytophotometrically as a function of position along an anterior-posterior (A-P) axis of the tissue throughout larval development. There was a dramatic 55-fold increase in the average amount of DNA per nucleus during the first 3 days of this period, but there was no further increase during the final day. However, the rate of increase had regional specificity. The amount of DNA per nucleus correlated significantly with its position along the A-P axis of the tissue: nuclei in a posterior direction contain gradually increasing amounts of DNA. There was up to a seven-fold difference between the smallest anterior and largest posterior nucleus. In addition, for three of the ages studied there was a subgradient in the posterior region with a slope that was considerably steeper than that of the overall tissue gradient. The tissue has three characteristic morphological regions, anterior, medial, and posterior, which can be recognized early in development and which are maintained throughout the larval period. The distribution of nuclear DNA classes determined for cells in each region for the final 2 days of larval life became fixed before the final day of development. The significance of the DNA gradient in terms of a protein storage gradient is discussed.