Myofiber expression of class I major histocompatibility complex accompanied by CD8+T-cell-associated myofiber injury in a case of canine polymyositis
Myofiber expression of class I major histocompatibility complex accompanied by CD8+T-cell-associated myofiber injury in a case of canine polymyositis
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DOI：
10.1354/vp.39-4-512
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发表时间：
2002-07-01
影响因子：
2.4
通讯作者：
Mabuchi, Y
中科院分区：
文献类型：
--
作者：
Morita, T;Shimada, A;Mabuchi, Y
一只7岁的雌性拉布拉多猎犬表现出极度的肌肉无力，肌肉萎缩。失语症和轻度吞咽困难。血清肌酸激酶升高（335 IU/l）。肌电图结果包括插入活动增加。颤动电位和奇怪的高频重复电位。骨骼肌组织病理学检查显示肌纤维坏死、吞噬、肌纤维再生和血管周围。肌周和肌内膜淋巴细胞、巨噬细胞和浆细胞浸润。免疫组化评价显示，肌炎早期浸润细胞为CD8+ t细胞，非坏死肌纤维表面明显增加了主要组织相容性复合体（MHC） I类的表达。重灾区CD3+细胞（T细胞）、hla - dr阳性巨噬细胞和B淋巴细胞较多。这些结果提示MHC I类表达和CD8+ t细胞浸润可能在肌炎的发生中起重要作用。这些组织病理学结果类似于人类自然发生的多发性肌炎。
A 7-year-old female Labrador Retriever dog showed extreme muscular weakness, muscle wasting. dysbasia, and mild dysphagia. An elevated value of creatine kinase (335 IU/liter) in the serum was detected. Electromyographic findings included increased insertional activity. fibrillation potentials, and bizarre high-frequency repetitive potentials. Histopathologic examination of skeletal muscles revealed myofiber necrosis and phagocytosis, regeneration of myofibers, and perivascular. perimysial, and endomysial infiltrations of lymphocytes, macrophages and plasma cells. Immunohistochemical evaluation demonstrated that infiltrative cells in the early stage of myositis were CD8+ T-cells and that an increased expression of major histocompatibility complex (MHC) class I was apparent on the surface of nonnecrotic muscle fibers. In contrast, many CD3+ cells (T cells) and HLA-DR-positive macrophages and B lymphocytes were found in the severely affected areas. These results suggest that both expression of MHC class I and CD8+ T-cell infiltration may play an important role in initiation of myositis. These histopathologic findings resemble those reported in naturally occurring polymyositis in humans.