IL-8 induces the epithelial-mesenchymal transition of renal cell carcinoma cells through the activation of AKT signaling
IL-8 induces the epithelial-mesenchymal transition of renal cell carcinoma cells through the activation of AKT signaling
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DOI：
10.3892/ol.2016.4900
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发表时间：
2016-09-01
期刊：
影响因子：
2.9
通讯作者：
Bi, Liangkuan
中科院分区：
文献类型：
--
作者：
Zhou, Nan;Lu, Fuding;Bi, Liangkuan
上皮-间质转化（EMT）过程由于其在人类肿瘤进展中的作用而越来越多地被研究。肾细胞癌（RCC）是导致患者死亡的最常见的泌尿系统肿瘤之一。以往的研究表明EMT过程与肾细胞癌的转移密切相关，但其分子机制尚未明确。本研究发现白细胞介素（IL）-8在转移性肾细胞癌中高表达。IL-8可通过增强N-cadherin的表达和降低E-cadherin的表达诱导肾癌细胞发生EMT。IL-8可诱导肾癌细胞AKT磷酸化，磷脂酰肌醇-4，5-二磷酸3-激酶抑制剂LY 294002可抑制IL-8诱导的肾癌细胞EMT。提示IL-8通过激活AKT信号转导通路促进肾细胞癌EMT，这可能为肾细胞癌转移提供了一种可能的分子机制。
The epithelial-mesenchymal transition (EMT) process has increasingly been examined due to its role in the progression of human tumors. Renal cell carcinoma (RCC) is one of the most common urological tumors that results in patient mortality. Previous studies have demonstrated that the EMT process is closely associated with the metastasis of RCC; however, the underlying molecular mechanism has not been determined yet. The present study revealed that interleukin (IL)-8 was highly expressed in metastatic RCC. IL-8 could induce the EMT of an RCC cell line by enhancing N-cadherin expression and decreasing E-cadherin expression. Furthermore, IL-8 could induce AKT phosphorylation, and the phosphatidylinositol-4,5-bisphosphate 3-kinase inhibitor LY294002 could inhibit the EMT of RCC cells that was induced by IL-8. Therefore, these results suggest that IL-8 is able to promote the EMT of RCC through the activation of the AKT signal transduction pathway, and this may provide a possible molecular mechanism for RCC metastasis.