Induction of invasive carcinomas in the accessory sex organs other than the ventral prostate of rats given 3,2'-dimethyl-4-aminobiphenyl and testosterone propionate.
Induction of invasive carcinomas in the accessory sex organs other than the ventral prostate of rats given 3,2'-dimethyl-4-aminobiphenyl and testosterone propionate.
复制标题
给予 3,2-二甲基-4-氨基联苯和丙酸睾酮的大鼠除腹侧前列腺外的副性器官中诱导侵袭性癌。
作者：
T. Shirai;S. Tamano;T. Kato;S. Iwasaki;S. Takahashi;N. Ito
用前列腺致癌物3，2 ′-二甲基-4-氨基联苯（DMAB）诱发F344大鼠前列腺癌，观察丙酸睾酮（TP）对前列腺癌发生的促进作用。一组动物接受s.c.以50 mg/kg体重的剂量注射DMAB，间隔2周，共沿着10次注射，同时皮下注射。含TP硅橡胶管的预处理。第二个实验组的大鼠以相同的剂量和间隔给予DMAB，但每次注射DMAB与之前连续3次每日100 mg/kg体重s.c.注射TP。停止给予致癌物后，这两组动物从第21周至实验结束接受TP植入物。在第56周处死所有存活动物，并将副性腺肿瘤发生率与DMAB单独给药组和其他适当对照组进行比较。给予TP + DMAB和随后长期给予TP的组发生了背外侧前列腺、精囊和前列腺的病变，这些病变都是浸润性腺癌。发生率分别为84.2%（16/19只大鼠）和66.7%（12/18只大鼠）。在13只动物中诱导了肉眼可见的大肿瘤，其中8只表现出转移到腹腔、肝或肺。除了给予TP注射加DMAB的组之外，没有一个对照组具有等同的肿瘤。腹侧前列腺癌的发展，这都是原位型，没有增加后续治疗TP。因此，这些数据清楚地表明，TP可以对背外侧前列腺、精囊和前列腺中的肿瘤发展产生强烈的增强作用，但在腹侧前列腺中不起作用。
The promotion effects of testosterone propionate (TP) on prostate carcinogenesis were investigated in F344 rats given the prostatic carcinogen, 3,2'-dimethyl-4-aminobiphenyl (DMAB). One group of animals received s.c. DMAB injections at a dose of 50 mg/kg body weight at 2-week intervals for a total of 10 injections along with s.c. implantations of TP-containing Silastic tubes. A second experimental group of rats was given DMAB at the same dose and intervals but each injection of DMAB was combined with 3 prior consecutive daily 100-mg/kg body weight s.c. injections of TP. After cessation of carcinogen administration, animals in these two groups received TP implants from week 21 to the end of the experiment. All surviving animals were killed at week 56 and accessory sex gland tumor incidences were compared to those in DMAB alone and other appropriate control groups. The groups given TP plus DMAB and subsequent long term administration of TP developed lesions of the dorsolateral prostate, seminal vesicles, and coagulating glands which were all invasive adenocarcinomas. Incidences were 84.2% (16 of 19 rats) and 66.7% (12 of 18 rats), respectively. Macroscopic large tumors were induced in 13 animals among which 8 demonstrated metastasis to the abdominal cavity, liver, or lung. None of the control groups except for the group given TP injections plus DMAB had equivalent tumors. Development of carcinomas of the ventral prostate, which were all of in situ type, were not increased by subsequent treatment with TP. These data thus clearly showed that TP can exert strong enhancing effects on tumor development in the dorsolateral prostate, seminal vesicles, and coagulating glands but not in the ventral prostate.