ACTIONS OF VASOPRESSIN AND ISOPRENALINE ON THE IONIC TRANSPORT ACROSS THE ISOLATED FROG-SKIN IN THE PRESENCE AND THE ABSENCE OF ADENYL-CYCLASE INHIBITOR-MDL12330A AND INHIBITOR-SQ22536
ACTIONS OF VASOPRESSIN AND ISOPRENALINE ON THE IONIC TRANSPORT ACROSS THE ISOLATED FROG-SKIN IN THE PRESENCE AND THE ABSENCE OF ADENYL-CYCLASE INHIBITOR-MDL12330A AND INHIBITOR-SQ22536
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DOI：
10.1016/0742-8413(91)90101-x
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发表时间：
1991-01-01
期刊：
影响因子：
--
通讯作者：
ARDIZZONE, C
中科院分区：
文献类型：
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作者：
LIPPE, C;ARDIZZONE, C
1. 研究了两种腺苷酸环化酶抑制剂（MDL12330A 和 SQ22536）对加压素和异丙肾上腺素诱导的青蛙皮肤离子转运的影响。2． MDL12330A 抑制血管加压素对短路电流 (SCC) 的作用，证实这种效应是 cAMP 介导的。3． 另一方面，异丙肾上腺素对 SCC 的作用不受 MDL12330A 的影响。 然而，这种缺乏作用并不能充分证明 cAMP 在此作用中的作用。事实上，由于MDL12330A也是cAMP磷酸二酯酶的抑制剂，这种作用可能掩盖药物对腺苷酸环化酶的抑制作用。4． 通过使用另一种腺苷酸环化酶抑制剂 (SQ22536)（可能对 cAMP 磷酸二酯酶没有作用），我们获得了异丙肾上腺素对 SCC 作用的强烈抑制作用。 因此我们得出结论，异丙肾上腺素对青蛙皮肤离子传输的作用也是 cAMP 介导的。
1. The effects of both adenyl cyclase inhibitors (MDL12330A and SQ22536) have been studied on the ionic transport induced by vasopressin and isoprenaline across the frog skin.2. MDL12330A inhibits the vasopressin action on the short-circuit current (SCC), confirming that this effect is cAMP-mediated.3. On the other hand, isoprenaline action on the SCC is unaffected by MDL12330A. However, this lack of effect is not a sufficient argument against the role of cAMP in this action; in fact, as MDL12330A is also an inhibitor of cAMP phosphodiesterase, this action could mask the inhibitory effect of the drug on adenyl cyclase.4. By using the other adenyl cyclase inhibitor (SQ22536), probably deprived of effect on the cAMP phosphodiesterase, we obtained a strong inhibition of isoprenaline action on the SCC. Thus we conclude that the actions of isoprenaline on the ionic transport across the frog skin are also cAMP-mediated.